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Regulation of Ethanol degradation in Acinetobacter baumannii
Taiwan (FMS-Taiwan) - Tzu Chi University, Hualien
Department of Microbiology
Dr. Nein-Tsung Lin
Dr. Guang-Huey Lin
Type of Research Project
- Basic science
What is the background of the project?
Acinetobacter baumannii is an important nasocomial pathogen in hosipital. Disinfection by 75 %enthanol in hospital is an general process, however residue ethanol will be degraded by Acinetobacter baumannii. It will cause the hospital aquired infection of this bacteria become a more severe problem than that the multidrug resistance of this bacteria.
What is the aim of the project?
Aim: Study gene regulation in bacteria.
What techniques and methods are used?
Used techniques: 1. Bacteria culture (mesophiles) 2. Two-dimensional gel electrophoresis. 3. RNA purification 4. Polymerase chain reaction (PCR) 4.Reverse transcription (RT)-PCR. 6.Genes cloning. 7. Sounthern hybridization. 8. Immunoflurecence detection of bacteria 9. Biofilm architecture analysis 10. Protein exprerssion . 11. Protein purification 12. Western blotting.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No specific skill will be required. There is no legal limitation for the students.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- G-H Lin; H-P Chen; H-Y Shu. Detoxification of indole by an indole-induced flavoprotein oxygenase from Acinetobacter baumannii. PLOSone 2015. e0138798
- H-Y Shu; L-C Lin; T-K Lin; H-P Chen; H-H Yang; K-C Peng; G-H Lin*. Transcriptional regulation of the iac locus from Acinetobacter baumannii by the phytohormone indole-3- acetic acid. Antonie van Leeuwenhoek. 2015(107):1237-1247.
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