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Determination of Pro-inflammatory Cytokine Interleukin-6 (IL-6) in Patients with Type 2 Diabetes Mellitus in Adult Population of Cosmopolitan Lahore, Pakistan
Pakistan (IFMSA-Pakistan) - Lahore medical and dental college, Lahore
Dr Rubina Bashir
Dr Umer Saeed
English : Required , Urdu : accepted
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Type 2 Diabetes mellitus is a clinical syndrome characterized by increased blood glucose levels due resistance to the action of insulin and an inability to produce sufficient insulin to overcome this ‘insulin resistance’. It is affecting people globally, and its prevalence in Pakistan is estimated to be around 11% adding to the national burden of diseases alarmingly. Some studies report the role of interleukin 6 (IL-6), a multifunctional cytokine, in the pathophysiology of type 2 diabetes mellitus. We are interested in further elucidating the role of IL-6 as a biomarker for diagnosis of type-2 diabetes and prognosis of treatment in patients with type-2 diabetes in Lahore, Pakistan.
What is the aim of the project?
To estimate the plasma levels of IL-6 in patients with type-2 diabetes mellitus and in healthy individuals using ELISA to determine any association of IL-6 levels with Type 2 diabetes mellitus.
What techniques and methods are used?
This study has been designed in a cross sectional manner using a non-probability sampling technique. The stages of the study are as followed; Initially, it involves collecting blood samples from Type 2 diabetic individuals (n=60) and healthy controls (=40) who are presenting to a single tertiary care hospital and from general population. 5ml of blood will be taken using gel vacutainer and will be conserved at 40°C. Then, the levels of Interleukin-6 will be detected in the laboratory using the Human ELISA* kits; (assay type with quantitative sandwich technique). Further the lab reports will be collected and processed into analytical software and data analysis would be performed using SPSS (Statistical Package for the Social Sciences) version 25 along with drawing interpretation of the results. Finally, a manuscript will be prepared in the IMRaD (Introduction, Methods, Results, and Discussion) format after due literature review. *enzyme-linked immunosorbent assay
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project is clinical
- the student will be allowed to work with patients
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be required to do an extensive literature search to gather sufficient knowledge and background information on the research area. He/she would also be required to withdraw blood samples from a fraction of the study population under supervision. Additionally, they would manage the preservation of the blood samples that were drawn using EDTA (Ethylenediaminetetraacetic acid) and ensuring integrity of the samples until handing them over to the laboratory. Alongside, he/she would observe ELISA (enzyme-linked immunosorbent assay) at the laboratory and learn its principles, materials and procedure of the test. Furthermore, the student will have to collect data from the lab reports online, enter data into SPSS sheets and analyze it to find a correlation between IL-6 and diabetes mellitus using appropriate statistical analysis tools. Lastly, they would interpret results after due discussions, develop a conclusion and prepare the manuscript as part of the research team. It must be noted that supervision will be provided to the student at most occasions.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
A 2 hour session on pathogenesis of diabetes mellitus and role of Interleukin 6 as mediator in the inflammatory cascade associated with Type 2 diabetes mellitus. In addition that a one hour session on basics of research methodology. All Sessions will be delivered by the mentor of the research project i.e Dr. Umer Saeed, Assistant Professor, Biochemistry Department, Lahore Medical and Dental College.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation - The student will prepare a scientific report - The student will prepare an abstract - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Good communication skills, basic knowledge of research methodology, scientific writing, Subjects passed : Biochemistry, Physiology
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
- Rehman K et al. Role of Interleukin-6 in Development of Insulin Resistance and Type 2 Diabetes Mellitus. Crit Rev Eukaryot Gene Expr. 2017;27(3):229-236. doi:10.1615/CritRevEukaryotGeneExpr.2017019712
- Kado S et al. Circulating levels of interleukin-6; its soluble receptor and interleukin-6/interleukin-6 receptor complexes in patients with type 2 diabetes mellitus. Acta Diabetol. 1999; 36:67–72 doi: 10.1007/s005920050147
- Akbari M; Hassan-Zadeh V. IL-6 signalling pathways and the development of type 2 diabetes. Inflammopharmacology. 2018;26(3):685-698. doi:10.1007/s10787-018-0458-0
- Malenica M et al. Importance of inflammatory markers and IL-6 for diagnosis and follow up of patients with type 2 diabetes mellitus. Med Glas (Zenica). 2017;14(2):169-175. doi:10.17392/920-17
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