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Potential protective role of nutritional molecules in cardiovascular and bone diseases: use of innovative cell culture models.
Università degli studi di Chieti
Department of Medical, Oral and Biotechnological Sciences University of Chieti “G. d’Annunzio” 31, Via dei Vestini – 66013 Chieti, Italy
English; Italian; Spanish; French
Type of Research Project
- Basic science
What is the background of the project?
Both cardiovascular complications and loss of bone mineralized matrix are associated to human aging and they are characterized by an increased susceptibility to heart and vascular disease and a decrease in bone mass and risk of fragility fractures, respectively. Evidence demonstrating a positive link between certain dietary patterns (e.g. Mediterranean Diet, caracterized by high consumption of fruits and vegetables rich in bioactive compounds and low consumption of “Junk Food”) and both cardiovascular and bone health, highlight an opportunity to investigate their potential to protect against the progress of cardiovascular complications and the deterioration of bone tissue during ageing. Although the list of nutritional molecules potentially playing a protective role on cardiovascular and bone health is extensive, evidence to define a clear link between these molecules and above diseases is currently insufficient to generate precise dietary recommendations. Moreover, some new nutritional molecules still need further investigation.
What is the aim of the project?
The main purpose of this project is to understand the potential efficacy of new nutritional molecules in maintaining well-being during aging, mainly related to cardiovascular system and bone.
What techniques and methods are used?
The following techniques and methods will be used: 1. Isolation and culture of endothelial cells, osteoblasts and different sources of stem cells from human tissues (adult and perinatal). Three-dimensional-cell culture system. 2. Protein extraction, preparation and purification, western blotting, immunocytochemistry, intracellular and extracellular staining for fluorescence microscopy, in vitro angiogenesis assay; Enzyme-Linked Immunosorbent Assay (ELISA); Flow cytometry. 3. DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) isolation and purification from cells and tissues; agarose gel electrophoresis; real-time polymerase chain reaction (RT-PCR). GraphPad prism, ImageJ, FlowJo and Microsoft Excel softwares will be used to analyze data.
What is the role of the student?
- The student will mainly observe
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
During the period of stay in the laboratory, the student will follow all the procedures relating to the isolation of the endothelial cells, the preparation of culture media and the maintenance of cells culture. The student will perform protein extraction, preparation and purification, western blotting, immunocytochemistry, intracellular and extracellular staining for fluorescence microscopy, in vitro angiogenesis assay, Enzyme-Linked Immunosorbent Assay (ELISA) and flow cytometry. The student will follow procedures for DNA and RNA isolation and purification from cells and tissues and subsequent agarose gel electrophoresis and RT-PCR. For the data analysis, the student will learn how to use GraphPad prism, ImageJ, FlowJo and Microsoft Excel.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There will be theoretical teaching provided by the tutors for the students through their participation to educative occasions such as laboratory meeting (once a week) in which will be discussed data, papers recently published and also will be the possibility to follow seminars taken from local and invited scientists.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The main skill required of the student is represented by his/her interest regarding the basic research and its potential translational value.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- 1. Mandatori D; Pipino C; Di Tomo P; Schiavone V; Ranieri A; Pantalone S; Di Silvestre S; Di Pietrantonio N; Ucci M; Palmerini C; Failli P; Di Pietro N*; Pandolfi A*. “Osteogenic transdifferentiation of vascular smooth muscle cells isolated from spontaneously hypertensive rats and potential menaquinone-4 inhibiting effect”. * Last two authors equally contributed to the work J Cell Physiol. 2019
- 2. Ucci M; Di Tomo P; Tritschler F; Cordone VGP; Lanuti P; Bologna G; Di Silvestre S; Di Pietro N; Pipino C; Mandatori D; Formoso G and Pandolfi A. "Anti-inflammatory role of carotenoids in endothelial cells derived from umbilical cord of women affected by gestational diabetes mellitus" Oxid Med Cell Longev. 2019
- 3. Mandatori D; Penolazzi L; Pipino C; Di Tomo P; Di Silvestre S; Di Pietro N; Trevisani S; Angelozzi M; Ucci M; Piva R; Pandolfi A. “Menaquinone-4 enhances osteogenic potential of human Amniotic Fluid Mesenchymal Stem Cells cultured in 2D and 3D dynamic culture system”. J Tissue Eng Regen Med. 2018
- 4. Castellano I; Di Tomo P; Di Pietro N; Mandatori D; Pipino C; Formoso G; Napolitano A; Palumbo A; Pandolfi A. “Anti-Inflammatory Activity of Marine Ovothiol A in an In Vitro Model of Endothelial Dysfunction Induced by Hyperglycemia”. Oxid Med Cell Longev. 2018
- 5. Di Tomo P; Di Silvestre S; Cordone VG; Giardinelli A; Faricelli B; Pipino C; Lanuti P; Peng T; Formoso G; Yang D; Arduini A; Chiarelli F; Pandolfi A; Di Pietro N. “Centella Asiatica and Lipoic Acid; or a combination thereof; inhibit monocyte adhesion to endothelial cells from umbilical cords of gestational diabetic women.” Nutr Metab Cardiovasc Dis. 2015
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