[Saltillo] Experimental model in rabbits of type 1 diabetes induced by Alloxan
Universidad Autonoma de Coahuila
Facultad de Medicina Unidad Saltillo Health Investigation Center Francisco Murguia 205, Zona Centro, 25000 Saltillo, Coah.
Alma Rosa Paredes Ramírez
Alma Rosa Paredes Ramírez
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No Yes Yes No No No No No Yes Yes Yes No
Type of Research Project
- Basic science
What is the background of the project?
Type 1 diabetes was known as "juvenile diabetes" or "insulin-dependent diabetes". A total insulin deficiency has been identified and is usually diagnosed in children and adolescents. Its etiology is the cause of the destruction of beta cells of the pancreas induced by an autoimmune effect, gene mutations or environmental factors. According to the International Diabetes Federation, 78,000 children develop type 1 diabetes each year. The annual incidence of type 1 diabetes has increased by around 3% in countries with high incomes, according to the World Health Organization. Due to this increase it is important to understand how this disease develops to subsequently find an appropriate treatment for it. With this, we want to study the different treatments and diagnostic methods in rabbits, so we can try to extrapolate the results to humans.
What is the aim of the project?
The aim of this project is to develop an experimental model of Diabetes Mellitus in rabbits using Alloxan and stablish the optimal dose of management for the induction of the illness for future studies.
What techniques and methods are used?
The study will be done in 15 New Zealand rabbits weighing more than 2.5 kilograms (females and males), divided randomly into 3 study groups Group I, Group II and Group III (control). It must be checked first that all animals are normoglycemic after 20 hours of fasting, in case any animal has alterations in glycemia, it will be discarded and replaced by another of the same characteristics. All the animals will be treated the same diet with 100 grams of rabbit food per day. On day 10 of its stay (after administration of Pentobarbital at 40 milligrams/kilograms of weight intravenously as an anesthetic), it will be given intravenous Alloxan 10% in a single dose at 110 milligrams/kilograms of weight (dissolved in 10 milliliters of injectable water) to Group I; Alloxan intravenous at 10% in single dose at 130 milligrams/kilograms of weight (dissolved in 10 milliliters of injectable water) to Group II; and, 10 milliliters of intravenous saline solution to Group III (control) with subsequent administration of 20% Dextrose during the first 24 hours intraperitoneal to each of the animals of the 3 groups. Strict monitoring of blood glucose variations will be carried out throughout the study. Slow-acting insulin will be administered subcutaneously to all the animals, starting at a dose of .3 units/kilogram of weight until the adjustment of a normal glycemia (approximately 1.1 units in total every 24 hours). Variables will be obtained: gender, preprandial glycemia, weight variations, variations in food and water intake, distal lesions in the extremities, qualitative glycosuria, qualitative proteinuria, glycemia and at the end anatomopathological analysis of pancreas and renal arteries. The gender will be defined by a veterinary. The glycemia will be taken by capillary glycemia. The weight will be taken by a weighing machine. Qualitative glycosuria and qualitative proteinuria will be taken by blood analysis. The anatomopathological analysis of pancreas and renal arteries will be taken by a biopsy. The data will be collected and analyzed using SPSS (statistical software system). Due to the use of Alloxan and the effects of diabetes mellitus in animals, all should be euthanized. According to the results of survival and behavior of the general state of the animals, control glycemia and anatomopathological study, the optimal dose of management for induction of diabetes in rabbits will be established as experimental models for future projects.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The student will measure preprandial and postprandial glycemia of the rabbit by using a glucose meter every third day for the first two weeks and once a week the following two, as well as helping the tutor with the administration of slow-acting insulin to the animal daily. The student will keep record of the glucose levels and the data collected will be analyzed with SPSS (statistical software system).
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- Rohilla; A.; & Shahjad; A. (2012). Alloxan Induced Diabetes: Mechanisms and Effects. International Journal of Research in Pharmaceutical and Biomedical Sciences; 3; 819-823. Obtenido de International Journal of Research in Pharmaceutical and Biomedical Sciences.
- Wen-Tao; S.; Chun-Ling; L.; Chun-Chao; B.; Zheng-Lan; C.; & Lu; Z. (2010). Effect of alloxan time administer Drug on establishing diabetic rabbit model. Obtenido de National Institutes of Health: 10.3980/j.issn.2222-3959.2010.03.04