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Role of MicrRNA in Infertility (Suez Canal University)
Egypt (IFMSA-Egypt) - Suez Canal University, Suez Canal
Dr. Mohamed Abdelwahed
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
MicroRNAs (miRNAs) are small non-coding single stranded RNA molecules of approximately 22 nucleotides that are physiologically produced in eukaryotic cells. In addition to the presence of miRNA at the cellular level, miRNAs have also been reported to be present in extracellular fluids such as plasma, saliva, vaginal fluids, menstrual blood, and semen. Abnormal expression of extracellular miRNA is attributed to different disorders, such as infertility. Infertility is defined as a disease of the reproductive system that is characterized by failure to achieve conception after 12 months or more of regular unprotected sexual intercourse (WHO, 2013). Approximately 15% of couples are infertile with males and females likewise suffering from infertility. In this study, we will use a miRNA microarray-based high throughput approach to identify and quantify the miRNA that was differentially expressed between the total RNA isolated from the normal semen from healthy males and the abnormal semen from infertile males with Non Obstructive Azoospermia (NOA) specifically the miRNA-145, and correlate the expression levels of this miRNA in seminal plasma of Non Obstructive Azoospermia (NOA) patients with clinical data and semen analysis of the patients.
What is the aim of the project?
To develop a new modalities for treating infertility due to Non Obstructive Azoospermia NOA by using the miRNA-145 in seminal plasma as a noninvasive diagnostic potential biomarker in Non Obstructive Azoospermia (NOA).
What techniques and methods are used?
A. Full history taking including Personal, Present, Past, Medical and fertility. B. Clinical examination as general examination, abdominal examination, Per rectal examination for the prostate and seminal vesicles and andrology measurements. C. Laboratory investigations as 1- Physical examination of the seminal fluid 2- Microscopic examination of seminal fluid to check fertility, Motility and shape of the sperms 3- Chemical examination for the seminal fluid to check the components and the PH (Power of hydrogen). 4- Assessment of miRNA-145 expression level in seminal plasma using the enzyme-linked immunosorbent assay ELISA.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Students are expected: 1- to have a part in history taking from the patient and this includes; a) personal history like name, age, occupation, marital state, address, drug abuse, etc. b) general history of any recent systemic illness or any complaint specific to the genitourinary system. c) medical history like history of diabetes, hypertension, hypothyroidism, renal or hepatic insufficiency d) surgical history focused on genitourinary surgeries. e) sexual history: history of sexually transmitted infections, erectile function, ejaculation, and libido; as these issues may affect fertility status. 2- to perform physical examinations including; a) general examination like measurement of body mass index, developments of secondary sex characteristics, the presence of gynecomastia or any abdominal scars. b) local examination: Evaluation of the following structures: the penis, scrotum, testes, epididymis, spermatic cord, vas deferens, and prostate. 3- to do and observe different lab investigations such as semen analysis or hormonal profile. 4- to assess miRNA-145 expression level in seminal plasma.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, the student will attend a lecture provided by the tutor explaining the study objectives of the project besides the methods and steps that the student will follow during the whole course to ensure that the student has a full background of the project.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Good communication skills and a full background of the projects needed for further examinations that will be done on the patients
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- • Abu-Halima M; Hammadeh M; Schmitt J; Leidinger P; Keller A; Meese E. Altered microRNA expression proﬁles of human spermatozoa in patients with different spermatogenic impairments. Fertil Steril 2013; 99: 1249–55.e16.
- • Antonio Luigi Pastore; Giovanni Palleschi; Luigi Silvestri; Antonino Leto and Antonio Carbone: Obstructive and Non-Obstructive Azoospermia; Male Infertility; Dr. Anu Bashamboo (Ed.); ISBN 2012: 978-953-51-0562-6.
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