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Incidence of Toxoplasmosis within slaughtered sheep and cattle in the north part of Palestine.
Palestine (PMSA) - Al-Najah National University, Nablus
Biomedical Sciences, Medicine and health sciences college
Dr: Walid Basha
Dr: Walid Basha
English and Arabic
Type of Research Project
- Basic science
What is the background of the project?
Toxoplasma gondii is an obligate, intracellular parasite, which is spread worldwide. The parasite is able to infect all warm-blooded hosts including humans.The infection occurs via consumption of food or water containing oocytes, eating undercooked meats containing tissue cysts, and placenta. Undercooked meat consumption is one of the most important ways of Toxoplasma transmission especially in pregnancy period. Raw and undercooked meats have been reported responsible for 50 % of congenital toxoplasmosis.
What is the aim of the project?
To determine the incidence of toxoplasmosis within slaughtered animals.
What techniques and methods are used?
Firstly, blood samples will be collected from slaughtered sheep by the doctor and will be given to the researchers. Secondly, the students will extract the DNA from the samples in the molecular lab of the university using DNA extraction solid phase extraction technique. Thirdly, the samples will be put in the gel electrophoresis to separate the DNA depending on their sizes and a control sample will be put too. Fourthly, researchers will observe the samples and see whether it contain toxoplasma or no. At the end, results will be documented and analyzed to conclude a result.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Students will actively participate in the collection and preparation of samples for DNA extraction. After the training process, the students will be able to extract the DNA from the samples and prepare the materials necessary for the PCR. After that students will be able to prepare the gel to separate the samples and determine the diagnostic points of Toxoplasma. Then the information and results will be analyzed and the students here will be able to see the prevalence of the microorganism in the sample as well as that they would be able to signify the result generally.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The training program will be performed by the Tutor for the basic techniques of DNA extraction and PCR plus about the basic concept of the research methodology.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation - The student’s name will be mentioned in a future publication - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
As there is a training program no specific skills will be needed.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- Thakur; R.; Sharma; R.; Aulakh; R.S. et al. Prevalence; molecular detection and risk factors investigation for the occurrence of Toxoplasma gondii in slaughter pigs in North India. BMC Vet Res 15; 431 (2019).
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