Projects
Name
Proteomic analysis of leukocytes
University
Slovakia (SloMSA) - University of Pavol Jozef Safarik, Košice
Domain
Biophysics and Medical Physics
Departement
Department of Medical and Clinical Biophysics
Head
Doc. RNDr. Ján Sabo, PhD
Tutor
Doc. RNDr. Ján Sabo, PhD
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes Yes No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
The project is focused on the proteomic analysis of the leukocytes of the patients with the breast cancer. The leukocytes are isolated from the blood sample of the patients. First sample is taken from the patients before any treatment and than next samples after each chemoterapeutic cycle. The samples are collected and divided into groups according to cancer stages and used chemoterapeutic treatment. Thereafter the proteins are isolated from the sample and both, protein identification and relative quantification is performed.
What is the aim of the project?
The project is focused on the proteomic analysis of the leukocytes of the patients with the breast cancer. The leukocytes are isolated from the blood sample of the patients. First sample is taken from the patients before any treatment and than next samples after each chemoterapeutic cycle. The samples are collected and divided into groups according to cancer stages and used chemoterapeutic treatment. Thereafter the proteins are isolated from the sample and both, protein identification and relative quantification is performed.
What techniques and methods are used?
What techniques and methods are used? Mention the steps/stages in this project. (Provide examples and avoid abbreviations; which tools will be used in general in this project?) Mainly used techniques for sample preparation are: centrifugation or ultracentrifugation, precipitation and fiter assisted sample preparation. For proteome analysis the bottom-up approach utilizing nano liquid chromatography combined with mass spectrometry are used. For data processing and evaluation several bioinformatic tools with different algorithms are used.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
What are the tasks expected to be accomplished by the student? (Minimum 10 lines, provide detailed explanation) Student will help with the clinical sample preparation (blood) for proteomic analysis. Sample preparation will consist of centrifugation/ultracentrifugation, protein precipitation utilizing acetone, protein sample purification (solid phase extraction, filtration membranes), protein digestion (trypsin or trypsin/LysC mix). Student will also assist during LC MS analysis (check the system operation conditions with the standard sample, set the measuring method).
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
none
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic experience in a lab work
Are there any legal limitations in the student’s involvement
No
Hours
8
Type of students accepted
This project accepts:
- Medical students
- Students in biomedical fields
Articles
- Henrik J. Johansson et al. Breast cancer quantitative proteome and proteogenomic landscape; Nature Communications 2019; 10:1600 | https://doi.org/10.1038/s41467-019- 09018-y
- BOBER; Peter et al. Proteomic analysis of the vitamin C effect on the doxorubicin cytotoxicity in the MCF 7 breast cancer cell line. In: Journal of Cancer research and Clinical Oncology. - ISSN 1432-1335. - Vol. 143; no. 1 (2017); s. 35 - 42.