Evaluation of antimicrobial resistance mechanism in Gram-negative bacteria
Universita degli Studi di Pavia
Department of Clinical-Surgical, Diagnostic and Pediatric Sciences, Microbiology and Clinical Microbiology Unit, via Brambilla 74, 27100, Pavia (PV)
Prof. Laura Pagani, Dr. Maria Sofia Caltagirone, Dr. Vittoria Mattioni Marchetti
Prof. Laura Pagani, Dr. Maria Sofia Caltagirone, Dr. Vittoria Mattioni Marchetti
English, Italian
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No Yes Yes No No No No No
Type of Research Project
- Clinical Project without Laboratory work
What is the background of the project?
The emergence of resistance to some or even all classes of antibiotics in previously susceptible bacterial pathogens is one of the most important challenges to infectious disease medicine. Antibiotic use in humans and animals carries implies an inherent risk of selecting for antimicrobial resistance genes through different mechanisms. In this scenario, the correct antibiotic susceptibility evaluation appears to be an essential premise to any antibiotic stewardship action. This is the reason why this project could be extremely useful for a medical student.
What is the aim of the project?
Detection of resistance mechanisms in Enterobacteriaceae, Gram-negative non fermenters and emerging opportunistic pathogens by phenotypic methods, accordingly to 2017 updated EUCAST guidelines. Antibiotic resistance, particularly to beta-lactams, will be investigated in all its many aspects: genetic, biochemical, molecular and clinical.
What techniques and methods are used?
1. study of nosocomial infections in high risk wards and molecular typing of micro-organisms aiming containment of epidemic episodes (by pulsed-field gel electrophoresis, multilocus sequence typing, etc). 2. evaluation of prevalence, spread and persistence of antibiotic-resistant microorganisms and/or clones (both in hospital and environmental setting). 3. correlation between antibiotic resistance and biofilm formation. 4. antimicrobial resistance genes identification using polymerase chain reaction, Microarray and sequencing. 5. plasmid characterization using PCR-based replicon typing kit.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be involved in all aspect of routine laboratory work (bibliographic research using online data bank, books and scientific journal; phenotypic and molecular tests execution; analysis of the results and explanation of all the steps of his activity). During the period of work in our department the student will also improve his skills in what regards teamwork and collaboration with different professional figures.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Learning speed, proper dexterity, good English skills Subjects passed: Microbiology Exams
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- 1. Caltagirone M et al.; Front Microbiol. 2017 Nov 10;8:2232. doi:10.3389/fmicb.2017.02232.
- 2. Nucleo E et al.; Antimicrob Resist Infect Control. 2018 Mar 6;7:33. doi:10.1186/s13756-018-0326-0.
- 3. Principe L et al.; Infect Drug Resist. 2018 Mar 9;11:377-385. doi:10.2147/IDR.S160489.