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Cell interactions in the tumor microenvironment and their pharmacological modulations
Slovakia (SloMSA) - University of Pavol Jozef Safarik, Košice
Department of Pharmacology
prof. DVM Ján Mojžiš, DSc.
prof. DVM Ján Mojžiš, DSc.
Type of Research Project
- Basic science
What is the background of the project?
Cancer cells are surrounded by a complex milieu. This cancer cell niche is called the tumor microenvironment (TME), and it contributes to the development and metastasis of tumors. The tumor microenvironment is a new emerging concept in tumor research and has become a research hallmark. TME is composed of cellular and non-cellular components (extracellular matrix - ECM). Multiple different cell types comprise the cellular compartment of the tumor microenvironment: (i) cells that are present in the normal tissue parenchyma before tumor development; (ii) cells that are recruited to the tumor-associated stroma from distal sites (i.e., the circulation or bone marrow). In the recent decades, considerable attention has been directed on cancer prevention and treatment by natural products. In this project we plan to study the effect of phytochemicals, mainly from the group of chalcones and indole phytoalexins, on the tumor-stroma interactions.
What is the aim of the project?
This project will be focused on describing the major types of tumor-stroma interactions and their modulation by newly-synthesized analogues of natural compounds.
What techniques and methods are used?
Cell cultures - primary human umbilical vein endothelial cells (HUVEC), human dermal microvascular endothelial cells (HMVEC-D), keratinocytes, primary fibroblasts isolated from skin and from tumor stroma, cancer cell lines (breast cancer cell lines - MCF-7 (the acronym of Michigan Cancer Foundation-7), MDA-MB-231) Flow cytometry, Western blot analysis, cell migration, cell proliferation, in vitro angiogenesis studies, immunohisto-chemistry Stage I. Cells interactions (interactions between stromal (dermal fibroblasts, cancer associated fibroblasts, endothelial cells) and epithelial cells (skin keratinocytes) and selected cancer cell lines (breast cancer cell lines - MCF-7, MDA-231), as well as between cells and ECM.) Stage II. Newly synthesized derivates of natural compounds and cell interactions
What is the role of the student?
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will participate in relatively simple methods for detection of cell proliferation and migration. Cell proliferation - MTT test (MTT - tetrazolium dye,dimethyltiazol) - rapid and widely accepted colorimetric assay for assessing cell metabolic activity BrdU (Bromodeoxyuridine) Cell Proliferation Assay - a non-isotopic immunoassay for quantification of BrdU incorporation into newly synthesized DNA (Deoxyribonucleid acid)of actively proliferating cells. Cell migration - the migratory activity either cancer or non-cancer cells will be assessed using a wound healing assay.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication - No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Students in biomedical fields
- Wu T; Dai Y: Tumor microenvironment and therapeutic response. Cancer Lett. 2017 Feb 28;387:61-68. doi: 10.1016/j.canlet.2016.01.043.
- Hui L; Chen Y: Tumor microenvironment: Sanctuary of the devil. Cancer Lett. 2015 Nov 1;368(1):7-13. doi: 10.1016/j.canlet.2015.07.039.
- Mirossay L; Varinská L; Mojžiš J. Antiangiogenic Effect of Flavonoids and Chalcones: An Update. Int J Mol Sci. 2017 Dec 22;19(1). pii: E27. doi: 10.3390/ijms19010027.
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