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Mutagenesis, genetic alterations, and genotoxic substances: their effect on cells.
Brazil (IFMSA-Brazil) - Universidade Estadual do Oeste do Paraná - Campus Francisco Beltrão (UNIOESTE FB), Francisco Beltrão
Laboratório de Microbiologia
Type of Research Project
- Basic science
What is the background of the project?
The study of the effects of genotoxic substances on organisms is essential because it has the ability to reveal changes in development, in the regulation of the cell cycle, in the identification of the processes of repair and cell control and maintenance of homeostasis. The fungus Aspergillus nidulans has been applied as a model in tests that seek to establish the effect of substances during genetic development and has been a promising resource for testing substances and agents of interest for nutrigenomics in the last 10 years. In this context, the objective of the project is to characterize the influence of nutritional factors, genotoxic and genoprotective agents in the germination process of Aspergillus nidulans conidia.
What is the aim of the project?
This project aims to evaluate the mutagenic and genotoxic effect of chemical and physical compounds on test organisms.
What techniques and methods are used?
Sterilization of laboratory materials by autoclaving. Preparation and handling of solid and liquid culture medium for microorganisms, including pH adjustment. Manipulation (eg, culture, inoculation and rejuvenation) of test organisms.Test group organisms will be exposed to agents that cause damage to genetic material, such as ultraviolet light (UV). Then, cell morphological analysis with optical microscopy and cell staining will be done to analyze deoxyribonucleic acid (DNA) damage. Finally, a comparison of test groups with the control group and data analysis, such as media and range deviation, is performed using the Microsoft Excel program.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student can develop various skills during the project with the help of the tutor. Major ones include laboratory organization and correct use of instrumentation and glassware, as well as proper sterilization by autoclaving. It will also develop practices regarding material and reagent handling, preparation of solutions (culture medium), handling of test organisms. Finally, it will develop the verification of genetic alterations through morphological analysis with microscopy, comparison between different strains and between test and control groups, as well as data analysis and quantification.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary readings will be given by the tutor and discussion of articles and projects in the area alongside other students, with a workload of up to 4 hours per week.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student must have good communication, initiative, and scientific and investigative spirit. Also, it is recommended that they are familiar with molecular biology techniques.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- Souza-Paccola; Edneia A.; et al. "Antimutagenic action of Lentinula edodes and Agaricus blazei on Aspergillus nidulans conidia." Brazilian Journal of Microbiology 35.4 (2004): 311-315.
- HOINATZ; K. ; ARRUDA; G. . Avaliação da toxicidade do extrato aquoso de Schinus terebinthifolius RADDI utilizando Allium cepa como bioindicador. Revista Científica JOPEF; v. 20; p. 178; 2015.
- ARRUDA; G.; et al. Phytochemical Prospecting; Antimicrobial Activity; and Acute Toxicity of Aqueous Plant Extract of Two Plant Species Allophylus edulis (A. St. Hilaire; Cambessedes & A. Jussieu) RADLK ex WARM and Matayba elaeagnoides RADLK. INTERNATIONAL JOURNAL OF NEW TECHNOLOGY AND RESEARCH; v. 5; p. 10; 2019
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