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Detection of respiratory pathogens in COPD at acute exacerbation and stable disease using molecular methods
Macedonia (MMSA) - St. Cyril and Methodius, Skopje
University clinic of pulmonlogy and allergology,
Prof. dr. Dejan Dokikj
Irina Angelovska, MD
English, Serbian, Croatian, Bulgarian
Type of Research Project
- Clinical Project without Laboratory work
What is the background of the project?
Chronic obstructive pulmonary disease (COPD) is the most common chronic disease in the adult population, and fourt cause of death around the world. The main cause for COPD is smoking, however other factors have been indetified which include: genetic predisposition, impaired growth and development and many environmental stimuli Because COPD is common, it is of upmost importance to know how to treat those patients and to know what may cause them further exacerbations. Because microorganism as omniprevalent and pneumonias are a common pathology, COPD patiant are at a grreater risk of having a severe exacerbation because of a respiratory pathoged. This is a reason why this project trie to detect these pathogens in patients with acute exacerbations of COPD
What is the aim of the project?
The aim ofthis project is to determine the respiratory pathogens (bacteria or viruses) during acute exacerbations and during the stable phases in COPD patients.
What techniques and methods are used?
Physical exam of patient including: body mass index, body tempereture, blood pressure and pulse. Analysis of patient’s sputum using a fast multiplex polymerase chain reaction (PCR) method for the detection of typical and atypical bacterial pathogens, and viruses. Chest X-ray will be done. Spiromethry of the patient to determine forced expiratory volume in 1st second (FEV1), forced vital capacity (FVC) and Tiffneau’s index.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project is clinical
- the student will take active part in the clinical examination
- If the project is clinical
- the student will be allowed to work with patients
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student is expected to learn the microorganisms which cause exacerbations of patients with COPD. The student will observe how the polymerase chain reaction machine works and how it is operated. The student is expected to learn the principles of PCR. The microorganisms which will be isolated using the fast multiplex PCR such as: Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis from the bacterial and Human rhinovirus, Influenza, Parainfluenza, Respiratory syncytial virus, Coronavirus и Adenovirus from the viral causes The results from all the beforementioned procedures will be coherently analyzed pertinent to the aim of the study and will be appropriately interpretated in order to find the most common respiratory pathogens asociated with acute COPD exacerbations
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Clinical students, able to obtain medical history, physical exam, basic lab interpretation.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
- Hurst JR; Vestbo J; Anzueto A; Locantore N; Müllerova H; Tal-Singer R; Miller B; Lomas DA; Agusti A; MacNee W; Calverley P. Susceptibility to exacerbation in chronic obstructive pulmonary disease. New England Journal of Medicine. 2010 Sep 16;363(12):1128-38.
- Papi A; Bellettato CM; Braccioni F; Romagnoli M; Casolari P; Caramori G; Fabbri LM; Johnston SL. Infections and airway inflammation in chronic obstructive pulmonary disease severe exacerbations. American journal of respiratory and critical care medicine. 2006 May 15;173(10):1114-21.
- Erb-Downward JR; Thompson DL; Han MK; Freeman CM; McCloskey L; Schmidt LA; Young VB; Toews GB; Curtis JL; Sundaram B; Martinez FJ. Analysis of the lung microbiome in the “healthy” smoker and in COPD. PloS one. 2011 Feb 22;6(2):e16384.
- Sethi S; Evans N; Grant BJ; Murphy TF. New strains of bacteria and exacerbations of chronic obstructive pulmonary disease. New England Journal of Medicine. 2002 Aug 15;347(7):465-71.
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