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"Study of serum vitamin D, vitamin B12, calcium, magnesium and phosphorous association in CKD patientsproject"
India (MSAI) - Parul Institute of Medical Sciences & Research, Vadodara, Gujrat
Dr. Garima Baradia
Dr. Garima Baradia
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Vitamin D is called the sunshine vitamin obtained from the UV rays of the sun. The vitamins functions in the body, in its active form. A healthy kidney has many vitamin D receptors which converts vitamin D into its active form. Activated Vitamin D helps in striking a balance between calcium and phosphorus levels in the body by controlling their absorption from the ingested food via Gastro-Intestinal Tract (GIT). Dialysis patients usually show hypo-hyper-vitaminosis for most vitamins, may be due to intradialytic loss of micro and macronutrients and malabsorption. When kidneys dysfunction, they lose the ability to activate vitamin D, which in turn leads to the derangement of calcium and phosphorus levels in the blood. In some patients undergoing dialysis a reversal in nerve conduction may be seen after supplementation of large doses of vitamin B12 Hyperphosphatemia has been consistently associated with Chronic Kidney Disease (CKD) progression. Some studies show less or even no association of calcium with the progression of CKD The goal of the study is to assay the levels of serum vitamin D, vitamin B12, calcium, phosphorous and magnesium in chronic kidney disease patients.
What is the aim of the project?
To detect the levels of serum vitamin D, vitamin B12, serum calcium, phosphorous and magnesium in CKD patients. To analyse the magnitude of vitamin D and magnesium insufficiency and deficiency in the above mentioned disease. To correlate the above parameters and associate them with the severity of the disease.
What techniques and methods are used?
It is a randomised case-controlled study to be performed on 25 Chronic Kidney Disease patients and 25 healthy controls. Subjects on any kind of medications, or vitamin supplementation will be excluded. Subjects, both males and females, above 18 years of age will be included. Both In-Patient Department (IPD) and Out-Patient Department (OPD) patients visiting the hospital will be enrolled in the project. CKD patients selected will be based on clinical history given to the clinician. A written informed consent will be taken from all the participants prior to their enrolment in the study. They will undergo complete systemic examination. Blood samples would be collected in plain vacutainer (with clot activator) under aseptic conditions for measuring serum levels of vitamin D (25(OH) D), vitamin B12, calcium, phosphorous and magnesium from all. Analytical Methods used for Serum vitamin 25(OH) D and vitamin B12 levels will be Chemiluminescent Immunoassay (CLIA) on Beckman Coulter, Access-2 instrument. Serum calcium, magnesium and phosphorous levels will be measured spectrophotometrically using ERBA-EM360 fully automated analyser. Statistical Methods: Results will be tabulated in MS excel sheet and statistical analyses will be carried out using the following methods: (1) Descriptive statistics to be calculated: for continuous variable range, mean (X) and standard deviation (SD) will be calculated and for categorical variable proportion percentage will be obtained. Comparison between two sample groups will be done using t-test and P values will be calculated. To compare between variables of three or more groups, Analysis of Variance (ANOVA) test was applied.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Study the Medical History taken by the clinician Observe the Blood sample collection To preserve and process the samples Centrifugation Pipetting through auto-pipettes To operate chemiluminescent analyser under observation To operate Erba-360 fully automated analyser under observation Data management Statistical analysis on STATA/IBM SPSS Presentation about the work done in conference Vit D & B12 by chemiluminescent analyser Erba-360 for Mg, Ca, Phosphorus. Our lab follows external quality control from Bio-Rad, the student will not be doing any quality control. He will only run standards along with the technical persons and observe and collect the values. Statistical analysis software STATA/IBM SPSS will be used
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary discussion on the Project will be done by Dr Garima.B and then Prof and HOD will elaborate and briefly explain the duties to be performed. Periodically 40 minutes briefing about the day’s activity will be discussed by Dr Garima.B on the project understanding, its requirements, method of sample collection and processing, patient dealing and disease information.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student’s name will be mentioned in a future publication - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Communicate in English Basic laboratory skills e.g. pipetting, Presentation skills
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Pre-Medical students from the American-British system - Students in biomedical fields
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