Definition of new therapeutic strategies based on the “re-education" of the microenvironment
Universita degli Studi di Udine
Istituto di Anatomia Patologica, Azienda Ospedaliera Universitaria di Udine, Ple S. Maria della Misericordia 15, 33100 Udine (Italy)
Prof Carla Di Loreto; Dr. Daniela Cesselli; Dr. Antonio Paolo Beltrami
Prof Carla Di Loreto; Dr. Daniela Cesselli; Dr. Antonio Paolo Beltrami
English, Italian
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes No No No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Translational medicine aims to transfer the advances in basic scientific research to define innovative approaches for the diagnosis and treatment of diseases. With this sp purpose, our research group is focused on the use of human adult stem cells isolated from human gliomas as an approach to personalize medicine and identify new prog and predictive factors of response to therapy and design new therapeutic strategies. In particular, the collaboration with neurosurgery led to the demonstration that in glio in addition to stem cells capable of starting the tumor (GSC), there is a population of stem cells, devoid of genetic mutations that characterize the tumor, but able to suppo growth of the tumor itself. These cells, called GASC (glioma-associated stem cells) are able, through the release of extracellular vesicles (e.g. exosomes), to increase the biological aggressiveness of the glioma-initiating cells. The project is part of a project entitled “Exosomes for regenerative, immunosuppressive, neuroprotective, and oncosuppressive terapie” (acronimo EXOTHERA), funded by the Interreg Program Italia-Austria 2014-20120.
What is the aim of the project?
The aim of the project is to develop new therapeutic strategies based on exosomes with the purpose of targeting the tumor microenvironment of human gliomas.
What techniques and methods are used?
We will use exosomes produced by human mesenchymal stem cells and/or cell of the tumor microenvironment as delivery systems of oncosuppressive signals. In particular, we will demonstrate whether it is possible to load Exosomes with drugs and/or miRNA/antagomir and will verify if they are able to act on the tutor supporting function of the tutor microenvironment and/or to kills cancer cells. The most promising therapeutic strategies will be tested in an animal model of orthotopic xenograft glioma, recently optimized in our group.)
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be included in a team already working in this project and will follow - for the time he/she will be in the lab - all the activities - including cell culture - micros - flow-cytometry - data collection - discussion of results with statistician - neurosurgeon and pathologist. Culture of stem cells from human gliomas In vitro assays to evalua drug sensitivity Characterization of cells by immunofluorescence and confocal microscopy. Flow-cytometry
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
At the beginning of the collaboration, the student will recieve papers and all the theroretical and practical information required for work in the project.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Student must be: 1. strongly committed to be involved in a research program including several basic-research activities and 2. able to work in a team.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Bourkoula E; et al. Glioma-associated stem cells: a novel class of tumor-supporting cells able to predict prognosis of human low-grade gliomas. Stem Cells. 2014;32(5):1 1253.