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In Vitro Activities of ceftolozane/tazobactam and comparable antimicrobial agents tested against Pseudomonas aeruginosa clinical isolates.
Turkey (TurkMSIC) - Akdeniz University, Antalya
Pinarbasi Mh. Konyaalti/Antalya , Akdeniz University Medical School Department of Medical Microbiology, Dumlupinar Blvd, 07070
Dr. Betil Ozhak
Dr. Gozde Ongut,Dr. Dilara Ogunc
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Pseudomonas is a clinically significant and opportunistic pathogen, often causing nosocomial infections. P aeruginosa is the second most common cause of nosocomial pneumonia (17%), third most common cause of urinary tract infection (7%), fourth most common cause of surgical-site infection (8%), and fifth most common isolate (9%) overall from all sites. In addition these organisms exhibit innate resistance to many antibiotics and can develop new resistance after exposure to antimicrobial agents. Intrinsic and acquired antibiotic resistance makes P. aeruginosa one of the most difficult organisms to treat. Ceftolozane is a semi synthetic cephalosporin of the 5th generation, and is indicated in the treatment of complicated nosocomial respiratory infections in combination with tazobactam, a beta-lactamase inhibitor. In this study we investigate the clinical efficiency of ceftolozane/tazobactam (Zerbaxa) against antipseudomonal antibiotics currenty in use; including carbapenems, ceftazidime, piperacilin/tazobactam, levofloxacin, ciprofloxacin and 4th generation cephalosporins. The study will be carried out on antibiogram plates containing microbiological isolates from patients with nosocomial p. Aeruguinosa infection.
What is the aim of the project?
The aim of this study was to investigate the in vitro antimicrobial susceptibility of Pseudomonas aeruginosa clinical isolates to ceftolozane/tazobactam and to see if a new therapeutic optio can be made available for this diificult to treat and fast adapting pathogen.
What techniques and methods are used?
Identification of the strains will perform using MALDI-TOF MS (In mass spectrometry matrix assisted laser desorption/ionization is an ionization technique that uses a laser a energy absorbing matrix to create ions from large molecules with minimal fragmentation) (Bruker Daltonics, Germany) according to the manufacturer’s instructions. -Antimicrobial susceptibility testing and interpretation of results will perform in accordance with EUCAST (European Committee of Antimicrobial Susceptibility Testing) recommendations. Antibiogram will be performed on p. Aeruguinosa selective Cetimide/Nalixidate agar. -Results will be catalogued in SPSS26 (statistical package for social sciences) for further biostatistical analysis.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- If the project is clinical
- the student will take active part in the clinical examination
- If the project is clinical
- the student will be allowed to work with patients
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The tasks expected of the student; -To learn about microbiological properties and resistance patterns of Pseudomonas aeruginosa - To observe and catalogue the results of the antibiograms to SPSS -To learn identification of the strains ill perform using MALDI-TOF. - To observe and ,if the student desires, assist doctors in obtainment of microbiological samples.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Lectures will be given twice a week by one of the tutors listed at the beginning, depending on their respective availabilities. Subjects covered will be; - Mechanisms of antimicrobial resistance - Pharmacology of antimicrobial agents - Clinical aspects and management options of p. Aeruguinosa infection - Prevention and management of nosocomial infections
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No specific skills required.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Dental medicine students (IADS members)
- Shortridge D; Pfaller MA; Arends SJR; Raddatz J; DePestel DD; Flamm RK. Comparison of the In Vitro Susceptibility of Ceftolozane-Tazobactam With the Cumulative Susceptibility Rates of Standard Antibiotic Combinations When Tested Against Pseudomonas aeruginosa From ICU Patients With Bloodstream Infections or Pneumonia. Open Forum Infect Dis. 2019;6(6):ofz240. Published 2019 May 20. doi:10.1093/ofid/ofz240
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