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Relationship between physical activity and cortisol levels
Brazil (IFMSA Brazil) - Centro Universitario CESUMAR (UNICESUMAR), Maringa
Department of Medicine
Marcel Pereira Rangel
Marcel Pereira Rangel
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
The lifestyle habits currently related to relationships with the development of metabolic syndromes, since, as people have less and less time for a healthy diet and suffer from stressful daily work agents, children and finances. recent studies show evidence that Temporomandibular disorder (TDM) linked to stress and increased cortisol, this may contribute to the development of a depressive disorder, provoking neuro-hormonal and immunological alterations, like hypercortisolemia causing a decline of neurotransmitters impairing the central serotonin system. Pharmacological treatment is very well established; however, it has adverse effects such as: weight gain, increased appetite, headache that limit its use. Thus, the association with physical activity is seen as a fundamental adjunct in the treatment, since individuals who practice physical activity increase the levels of fundamental neurotransmitters in the treatment of depressive symptoms, as well as, reduce cortisol levels. In this way, they help in the treatment of depressive disorders, in addition to reducing cortisol levels, helping as a protective factor in development behavioral, metabolic or sleep-related problems.
What is the aim of the project?
The aim of the project is to relate the benefits of exercising to cortisol levels.
What techniques and methods are used?
The research will meet all ethical precepts according to resolution 466 of 2012, passing through the evaluation and approval of the Committee of Ethics and Research with Human Beings (CEP) of the University Center of Maringá University (UniCesumar). All participants will be informed by reading and signing the Informed Consent Form (TCLE). This is an exploratory descriptive study of cross-sectional design and quantitative approach. The study population will be composed by people located in the city of Maringá-PR. Samples will be collected within 30 days after approval of the research ethics committee, with project durantion of 8 weeks. In this investigation the following evaluation tools will be used: 1- Identification. It will be carried out through data collection such as: Age, sex, weight, height, lifestyle: Practice of physical activity, use of alcohol, food, etc. 2- Application of the Pittsburgh Sleep Quality Index” questionnaire, evaluates the quality and quantity of sleep and its general aspects, as well as the presence of sleep disorders. 3- Hormonal dosage (cortisol level) blood will be collected to determine plasma cortisol levels by an immunoenzymatic method (ELISA). The students participating in the study will measure the hormones cortisol in three moments: beginning of the academic semester, middle (before the evaluations) and at the end of it. Blood collection materials will be needles, syringes, cotton, garrote, gloves, post-puncture dressings and alcohol. The blood of each one will be collected in the Clinical Unit of the Institution in the morning, between seven and ten hours, after respecting the fast of eight hours. After collection, the blood will be centrifuged and stored at -8ºC before dosing. The analysis of cortisol levels will be performed by the method of electrochemiluminescence (ELISA) with the kit from the USA DIAGNOSTICA brand, being analyzed in BIOPLUS 2000 a high performance biochemistry system. The equipment is a photometer that makes measurements of radiant energy transmitted, absorbed, dispersed or reflected under controlled conditions 4- Data analysis - Descriptive analysis will be performed with univariate frequency tables, with percentage distributions for qualitative variables and with the calculation of central tendency measures such as medians, means and standard deviations for quantitative variables. The measure of proportionality adopted will be the prevalence ratio (PR), which is a substitute for relative risk (RR), generally estimated in cross-sectional studies (ALMEIDA FILHO; ROUQUAYROL, 2006). The measure of significance observed will be the chi-square association test for qualitative variables, and when appropriate, Fisher's exact probability test. Student's t test will be used for quantitative variables, observing a significance level of 5% in all analyzes. 5- Risks: Individuals participating in the project will be subject to risks related to the collection of biological material and application of the questionnaire. Blood collection provides a procedure of great importance for research, however, it can also be a process with risks to participants, however, these risks will be minimized by researchers, since blood collection will be carried out in an appropriate place with all patients. personal protective equipment by the collector minimizing contamination risks for patients such as (Glove, syringe, needle, tubes with sterile anticoagulants), the collection procedure will follow the recommendations of the WHO Guidelines for drawing blood: good practices in phlebotomy. The applications of the questionnaires provide a simple procedure, however, discomfort may occur at the time of application (tension, discomfort, etc.), to minimize these aspects, the researchers will apply the questionnaires in an open, wide place, with comfortable chairs and without noise, to make the individual as comfortable as possible and without pressure to finish the answers. PATIENTS INCLUSION Respect Fasting Accept and Sign the TCLE EXCLUSION PATIENTS - Present some condition that interferes with the collection of data. - Do not respect Fasting;
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- If the project is clinical
- the student will take active part in the clinical examination
- If the project is clinical
- the student will be allowed to work with patients
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
1. STATISTICAL ANALYSIS - The results will be analyzed by the statistical method PERMANOVA, through the statistical program STATISTICA (6,0). 2. BLOOD COLLECTION/QUESTIONNAIRES - Questionnaires will be approved along with blood collections, or the student will be able to perform a blood collection following the phlebotomy steps recommended by the World Health Organization, as well as, multiple choice applicators or questionnaires, where the information as collected will be used in the statistical analysis process. 3. CLINICAL EXAMS –Biochemical tests will be carried out in the clinical analysis laboratory of Unicesumar (UNICESULAB), where it has a physical structure and equipment capable of carrying out the dosages recommended in the project. In this step, the supervisor will explain all the steps to the student so that together they can carry out the planned analyzes. 4. SPECIFIC READING - Review with research in the database relevant to the theme throughout the study period, for the formation of the questionnaire and the base text. The advisor will also assist the student in researching articles for making and publishing the article. 5. PUBLICATION -The results will be analyzed and discussed according to the standard of the journal pre-selected by the project participants. Submission will be given to a pre-selected magazine by the project participants, with a minimum impact on Qualis B3
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student after approval in the exchange process, can contact the tutor directly so that a basic reading is sent, as a way of prior learning for all the project processes. The tutor will also be available for meeting with the student whenever needed.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Proactive, Committed, Interested
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- DuBose K; Makune A. The relationship between objectively measured physical activity; salivary cortisol; and the metabolic syndrome score in girls. Pediatr Exerc Sci. 2014 Aug;26(3):221-30. (https://www.ncbi.nlm.nih.gov/pubmed/24722755)
- Casazza K; Dulin-Keita A; Gower B; et al. Differential influence of diet and physical activity on components of metabolic syndrome in a multiethnic sample of children. J Am Diet Assoc. 2009 Feb;109(2):236-44.
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