Projects
Name
Analysis of Mitochondrial Functions in Cell Culture Aging Model
University
Turkey (TurkMSIC) - Dumlupinar University, Kutahya
Domain
Histology
Departement
Kütahya Health Sciences University Medical Faculty. Department of Histology and Embryology, Kutahya Health Sciences University Evliya Celebi Campus, Tavsanli Road 10th km. Kutahya
Head
Dr. Suna SAYGILI
Tutor
Dr. Ahmet KOCAK
Languages
Turkish, English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No Yes No No No No No No No
Type of Research Project
- Basic science
What is the background of the project?
Mitochondrion is the powerhouse of the cell, and is inseperable from the everyday metabolism of any and every eukaryote. It is also established that mitochondria have life cycles and have associated molecules with respective effects on the whole of the cell. Mitochondria, however, also produce radical oxygen products (ROS) which are known to form chemical bonds at random with various cellular structures, damaging and aging the cells they’re within. Our research will focus on the effects of mitochondrial functionality on cellular longevity. We will investigate the effects of different levels of mitoconhdrial efficiency on cellular lifespan. We will simulate different levels of mitochondrial functionality in-vitro by subjecting cells to dinitrophenol (DNP, an agent that inhibits mitochondrial generation of ATP) at different levels and observe the longevity of culture lines. By doing this we may obtain an ‘’optimal’’ mitochondrial output level for continued cellular function, which may help open new avenues of research to help guide ageing related research in the future.
What is the aim of the project?
To determine whether there is an ‘’Optimal’’ level of mitochondrial activity for optimal cellular longevity, which can help open up new possibilities in ageing research via mitochondrial function.
What techniques and methods are used?
The outline of the research is as follows; - Cell cultures of non-immortalized ephitelial lines will be seperated into control and DNP groups with different concentrations - After incubation with DNP injection into the medium, cultures will be examined under light microscopy and SEM (scanning electron microscopy) for signs of degeneration at different intervals - Results will be entered and processed with SPSS 26 (statistical package for social sciences)
What is the role of the student?
- The student will mainly observe
- The tasks of the student will be performed on his/her own
What are the tasks expected to be accomplished by the student?
The student will mainly observe the procedures and will discuss the results and notes at the end of the day with the tutors. Specific tasks may include: - Using light microscopy - Handling cell cultures (preparing media solutions, helping operate incubators etc) - Observe (but not participate in) the SEM imaging of cultures - Observe the infusing of cultures with the DNP solution. - Help chekc if DNP concentrations are accurate via spectrometry - Attend daily discusions ith the lab staff about the days proceedings each morning
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No scheduled lectures will be given but the tutors will be with the student constantly to explain both the technicalities and the science behind the procedures in the project; as well as mechanisms of cellular aging. The teaching will be provided by Dr. Ahmet Kocak.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No specific skills are required.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Students in biomedical fields
- Dental medicine students (IADS members)
Articles
- Nuo Sun;1 Richard J. Youle;2 and Toren Finkel1 The Mitochondrial Basis of Aging; PMCID: PMC4779179 NIHMSID: NIHMS757278 PMID: 26942670; Mol Cell. 2016 Mar 3; 61(5): 654–666. doi: 10.1016/j.molcel.2016.01.028
- Edward J. Lesnefsky;1;2 Qun Chen;1 and Charles L. Hoppel3;4;5;*; Mitochondrial Metabolism in Aging Heart; PMCID: PMC5009371 NIHMSID: NIHMS779394 PMID: 27174952; Circ Res. 2016 May 13; 118(10): 1593–1611. doi: 10.1161/CIRCRESAHA.116.307505