PKA expression differences between normal sperm samples and oligoasthrozoospermic samples.
Turkey (TurkMSIC) - Istanbul Medipol University , Istanbul
Istanbul Medipol University School of Medicine Urology Hospital TEM Avrupa Motorway Goztepe Exit No: 1 Bagcilar 34214 Istanbul
Seda Karabulut (Assist. Prof. Dr.)
Seda Karabulut (Assist. Prof. Dr.)
Turkish, English
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes No No No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Protein Kinase A (PKA) is a cellular signalling protein that acts as the end point molecule of the cAMP (cyclic adenosine monophosphate) guided G-Protein signalling pathway. cAMP-PKA coupled signalling, also known as ‘’Gs(stimulant) pathway’’ plays a pivotal role in almost all cellular signalling that involves activation of a cell’s specific functions such as; neuronal signalling, cellular motility, immune cell activation and endothelial function. Infertility is defined as the inability to conceive within one year of planned, regular, unprotected intercourse. Nearly half of the infertility cases have their origin in the male partner’s genitourinary function. Spermatic causes of male infertility can be bluntly grouped under ‘’oligozoospermia’’ (low number of sperm) and ‘’asthenozoospermia’’ (sperm incapable of movement). Oligoasthenozoospermia is a cause of severe male infertility defined by low numbers of immotile sperm cells. Our research investigates whether there is any statistically significant difference of PKA expression between control and oligoasthenozoospermic samples. If there is any difference between the groups, it may open up a novel treatment target for this disease.
What is the aim of the project?
To investigate the difference of expression of PKA between healthy and oligoasthenozoospermic samples to see if PKA might have any role in the pathogenesis of male infertility.
What techniques and methods are used?
The project will use the following methodologies: - Establishing baseline sperm counts and motilities of all experiment groups via light microscopy - Seeing if there is any visible difference in sperm motility using immunohistochemical staining with PKA-specific antibody dyes. - Putting sperm samples with different cellular densities and motility levels through Western Blotting and PCR (polymerase chain reaction) to see their respective PKA expression and genetic availabilities - Catalouging the obtained data using SPSS26 (statistical package for social sciences)
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student is expected mainly to carefully observe the described procedures and discuss the activities at the end of the day with the tutor. Specific tasks may include; - Determining sperm counts with light microscopy - Helping run the PCR and subsequent gel electrophoresis (aka. The Western Blot procedure) - Entering data to archives using SPSS and Microsoft Excel
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Lectures will be scheduled according to the availability of Dr. Seda Karabulut. Subjects will include; - Spermatogenesis, oogenesis and fertilization - Pathogenesis, treatment options and classification of human infertility
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No specific skills are required.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Genetics of male infertility. Krausz C; Riera-Escamilla A. Nat Rev Urol. 2018 Jun;15(6):369-384. doi: 10.1038/s41585-018-0003-3. Review. PMID: 29622783
- RNASET2 impairs the sperm motility via PKA/PI3K/calcium signal pathways. Xu Y; Fan Y; Fan W; Jing J; Xue K; Zhang X; Ye B; Ji Y; Liu Y; Ding Z. Reproduction. 2018 Apr;155(4):383-392. doi: 10.1530/REP-17-0746. PMID:29581387