Projects
Name
Microbiology investigations and studies in Karatay University Department of Microbiology
University
Turkey (TurkMSIC) – Karatay University LC, Konya
Domain
Microbiology
Departement
Konya Ticaret Odasi Karatay University, Faculty of Medicine, Department of Microbiology, Akabe, Alaaddin Kap Cd. No:130, 42020 Karatay/Konya
Head
Asst.Prof. Dr. Merve Aydin Terzioglu
Tutor
Research Assistant Tugce Duran
Languages
Turkish, English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No No Yes No No No No
Type of Research Project
- Basic science
What is the background of the project?
Isolated organ preparations are often used to evaluate the muscle-relaxant effect of the research substance on tissue. The organ bath systems used for this purpose allow for the investigation of a large number of substances in smooth muscle tissue. We also perform cell culture, western blot, real time PCR (Polimerase Chain Reaction) and standard simple microscopic examinations in our laboratory. The aim of cell culture studies; The aim of this study is to provide researchers with as many experimental and experimental cells as possible in vitro, similar to the function of almost every tissue. Of Turkey to the Medical Faculty of Medical Biology Department of a group of faculty members in the Department, held in Antalya in 1996, "Medical Biology Congress" in on the premise that the establishment of an association to achieve the purpose above, Istanbul Faculty of Medicine at the meeting, Medical Biology Department Chairman Prof. Dr. Mahmut Çarin has undertaken to establish this association. The association was established in Istanbul on 25.07.1997 under the name of “Medical Biology Association“. The Association was established to gather the researchers working in the field of Medical Biology and Genetics, to provide a scientific cooperation and cooperation environment, to organize the meetings and to transfer the progress made to the scientific world.
What is the aim of the project?
The aim of this program is to optimize cell culture techniques, western blot analyses, real time PCR and isolated organ studies techniques together with tutors.
What techniques and methods are used?
Agarose gel electrophoresis and Western Blot that are used. SDS polyacrylamide gel electrophoresis (SDS-PAGE) immediately can be applied in the analysis of all protein types. -Cell culture techniques like ;passaging, thawing and freezing - Real-time PCR (RT-PCR) is also called quantitative PCR or qPCR. The key feature in RT-PCR is that amplification of Deoxyribo Nucleic Acid (DNA) is detected in real time as PCR is in progress by the use of fluorescent reporter. The fluorescent reporter signal strength is directly proportional to the number of amplified DNA molecules. -Basic microscope using techniques and types - Isolated organ bath techniques
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The following tasks will be expected to be accomplished by the student: -Learning isolated organ tissue techniques - Learning cell culture protocols, passaging, thawing and freezing - Usage and applying of the PCR method with the help of the tutor (polymerase chain reaction) which is a method widely used in molecular biology to make several copies of a specific DNA segment amplifications. - Learning Western Blotting techniques - Learning Gel electrophoresis types Will the student learn and perform those techniques? Will any data analysis be performed? Will any specific software be used?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There are many different subjects open for understanding methods.The subject will be taught once per week by tutor Assistant Prof. Merve Aydın Terzioglu and Research Assistant Tugce Duran. -Cell Cycles -Principles of Real Time Polymerase Chain Reaction (RT-PCR) -Isolated organ -Cell Culture Protocols - Gel electrophoresis working principle -Steps of Western Blotting and analyses
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No specific skills are required.
Are there any legal limitations in the student’s involvement
No
Hours
8
Type of students accepted
This project accepts:
- Medical students
Articles
- Good Cell Culture Practice for stem cells and stem-cell-derived models.Pamies D; Bal-Price A; Simeonov A; Tagle D; Allen D; Gerhold D; Yin D; Pistollato F; Inutsuka T; Sullivan K; Stacey G; Salem H; Leist M; Daneshian M; Vemuri MC; McFarland R; Coecke S; Fitzpatrick SC; Lakshmipathy U; Mack A; Wang WB; Yamazaki D; Sekino Y; Kanda Y; Smirnova L; Hartung T. DOI:10.14573/altex.1607121
- Isolation; Culture; and Expansion of Mesenchymal Stem Cells.Ferrin I; Beloqui I; Zabaleta L; Salcedo JM; Trigueros C; Martin AG. DOI:10.1007/978-1-4939-6921-0_13
- Western blotting.Biji T.KurienaR. HalScofield https://doi.org/10.1016/j.ymeth.2005.11.007