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Early urinary biomarkers for renal lesion in obese children and adolescents
Macedonia (MMSA) - St. Cyril and Methodius, Skopje
Institute of Medical and Experimental Biochemistry
Prof. dr. Jasna Bogdanska
Prof. dr. Svetlana Cekovska
English, Serbian, Croatian, Bulgarian
Type of Research Project
- Basic science
What is the background of the project?
Obesity in children and adolescents is a global epidemiological problem that is linked to essential hypertension and diabetes type 2 – leading causes of end- stage renal disease. The global prevalence of obesity grew from less than 1% to 6-8% among boys and girls, 3% to 11% among men, and 6% to 15% among women between 1975 and 2016.2 Increased prevalence of obesity was followed by increased prevalence of hypertension, diabetes, and cardiovascular disease. The adverse effects of hypertension and peripheral insulin resistance coupled with systemic inflammation and dyslipidemia may trigger the development of chronic kidney disease (CKD) which we will try to forsee.
What is the aim of the project?
The aim of the project is to find the most sensitive and promising biomarkers for detection of early-stage kidney lesion in obese children and adolescents, and use them in another relevant prospective study.
What techniques and methods are used?
This project will include 100 obese children and adolescents aging from 5 to 19, with the equal prevalence of both males and females. Control group will include 50 healthy children and adolescents. Data Analysis – Body mass, height, blood pressure, gender Calculation of Body Mass Index (BMI) – children and adolscents with BMI over 30kg/m 2 will be included in this research project Laboratory Analysis – determination of differential blood count via hematological analyzer, calculation of serum concentrations of urea, creatinin, uric acid, glucose cholesterol, triglicerides , high-density and low-density lipoproteins cholesterol, using standard spectrophotometric methods, as well as calculation of insulin concentration using Enzyme Chemiluminiscence Immuno Assay (ECLIA) method. Specific Renal Biomarkers – determination of creatinin concentration, microalbumin, activity of N-acetyl beta-D- glucosaminidase (NAG) enzyme, concentration of kidney- injury molecue-1 Sodium Dodecyl Sulphate Poliacrilamid Gel Electrophoresis (SDS-PAGE) – 3 urine samples of each patient will be analyzed with SDS-PAGE (first morning urine, second urine and urine sample after excercise). Turbidimetry – will be used for microalbumin concentration analysis Enzyme Linked Immunosorbent Assay (ELISA) – will be used for calculation of kidney-injury molecule-1 concentration
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
What are the tasks expected to be accomplished by the student?
The student is expected to learn the methods used for analysis of important kidney lesion biomarkers, which are determination of creatinin concentration, microalbumin, activity of N-acetyl beta-D- glucosaminidase (NAG) enzyme, concentration of kidney-injury molecue-1. The student will perform laboratory tasks such a pipeting, performing SDS- PAGE, and placing material for ELISA. After the data from the analysis is collected, the student is supposed to analyze the data regarding the degree of kidney injury if present, and afterwards to form a conclusion and evaluation based upon statistical analysis.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, the tutor will provide both theoretical and practical knowledge.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Devotion and commitment to research medicine
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- Jastreboff AM ; Kotz CM; Kahan S; Kelly AS; Heymsfield SB. Obesity as a Disease: The Obesity Society 2018 Position Statement. Obesity (Silver Spring) 2019;27:7-9. [PubMed]Jastreboff AM; Kotz CM; Kahan S; Kelly AS; Heymsfield SB. Obesity as a Disease: The Obesity Society 2018 Position Statement. Obesity (Silver Spring) 2019;27:7–9.
- Whaley-Connell A; Sowers JR. Obesity and kidney disease: from population to basic science and the search for new therapeutic targets. Kidney Int 2017;92:313-23. [PubMed]Whaley-Connell A; Sowers JR. Obesity and kidney disease: from population to basic science and the search for new therapeutic targets. Kidney Int. 2017;92:313–323.
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