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(Sibiu) Quality control for disk diffusion method for antimicrobial susceptibility testing
Romania (FASMR) - "Lucian Blaga" University - Faculty of Medicine, Sibiu
Dr. Teodora Calin
Dr. Teodora Calin, Dr. Geta Hilma
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Due to their specificity of action, antibiotics show different efficacy compared to multiple microbial species. Even within the same microbial family, there may be large differences in sensitivity of different strains to a particular antibiotic, so establishing antibiotic treatment in the clinic requires isolating the microbial strain and determining its spectrum of antibiotic sensitivity. There are a lot of different microbial resistance testing methods, but with the help of the European Union funds and standardisation of different hospital laboratory testing, disk diffusion method will gain prevalence in becoming the new standard for testing and discovering antibiotic resistance in new strains of bacteria With the support of European countries, EUCAST (European Committee on Antimicrobial Susceptibility Testing) has developed a disk diffusion test with zone diameter breakpoints. The development of the EUCAST disk diffusion method and quality control criteria are described, together with guidance on quality control and implementation of the method in clinical microbiology laboratories. The method includes the use of Mueller–Hinton agar medium.
What is the aim of the project?
The aim of the project is to update different antimicrobial testing methods to the highly accurate disk diffusion test commissioned by EUCAST in order to create a new standard for antimicrobial resistance prevalence.
What techniques and methods are used?
What techniques and methods are used? Mention the steps/stages in this project. (Provide examples and avoid abbreviations; which tools will be used in general in this project?) The project is meant for the student to achieve theoretical and practical information about creating antibiograms. Therefore, he/she will cultivate cell culture dishes, reconstruct reference strains and verify the purity of the fraction and microscopic examination. The student will prepare bacterial inoculum, cultivate board. After these steps are completed, microtablets of antibiotics will be disposed on the board. The board will be incubated and examined after incubation. The quality control will be done for 20 days and the student will register the changes, make charts, analyse the errors and correct them.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
What are the tasks expected to be accomplished by the student? (Minimum 10 lines, provide detailed explanation) The student will - cultivate cell culture dishes - reconstruct reference strains - verify the purity of the culture- fractions and microscopical examination - prepare bacterial inoculum - cultivate board - dispose microtablets of antibiotics on the board - incubate and examine the boards after incubation - quality control after 20 days according to strains of reference - analyse the errors and correct them The activity will be registered in an Excel chart
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The local committee will provide an introduction course into research techniques and the tutor will provide further bibliography upon arrival.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
What skills are required of the student? - To be able to use the microscope - To have general knowledge in the subjects of: microbiology, pharmacology, immunology Subjects passed: microbiology
Are there any legal limitations in the student’s involvement
If yes, what are the limitations? The students will need to sign papers regarding laboratory data confidentiality and a general accord from the hospital internal regulation. The students will need to receive a lecture about staff safety and health because they will be working with live potentially dangerous bacteria. All these forms will be translated from Romanian to English.
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
- Matuscheka E; Brownb D; Kahlmetera G. Development of the EUCAST disk diffusion antimicrobial susceptibility testing method and its implementation in routine microbiology laboratories. Clinical Microbiology and Infection 2014;20;4;O255-O266
- HM Ericsson; JC Sherris. Antibiotic sensitivity testing. Report of an international collaborative study. Acta Pathol Microbiol Scand B Microbiol Immunol; 1971; 1
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