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(Cluj-Napoca) Antioxidant and anti-inflammatory effects of plant extracts
Romania (FASMR) - University of Medicine and Pharmacy "Iuliu Hatieganu", Cluj-Napoca
Tutor(s) Alina Elena Parvu
Tutor(s) Alina Elena Parvu
Type of Research Project
- Basic science
What is the background of the project?
Natural products, such as plants extract, either as pure compounds or as standardized extracts provide unlimited opportunities for new drug discoveries because of the unmatched availability of chemical diversity. Various nonsteroidal anti-inflammatory drugs have been shown to reduce pain and inflammation by blocking the metabolism of different enzymes involved, thereby reducing the production of inflammatory cytokines. Sadly, there are many side effects associated with the administration of nonsteroidal anti-inflammatory drugs. However, there are medicinal plants with both anti-inflammatory and antioxidant therapeutic effects with low or no side effects, as well as antioxidant. Plant tissues contain a wide variety of compounds with antioxidant activity, such as flavonoids, lignans, alkaloids, terpenes and others. Some of them have the ability to protect against enzymatic and nonenzymatic lipid peroxidation in model membranes.
What is the aim of the project?
The aim of the research is to test what kind of antioxidant and anti-inflammatory effects do plant extracts have on living organisms, by testing them in vitro and in vivo.
What techniques and methods are used?
The first step is inducing experimental inflammation in rats, after which the animals are treated with plant extracts. The next step is performing biochemical tests for oxidative stress and inflammation evaluation: total oxidative status, total antioxidant capacity, oxidative stress index, nitric oxide, total thiols and malondialdehyde. Finally, the student performs statistical analysis of the results. The results will be compared with other achievements from previous studies and discussed with the head of the department.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The students will work both in vitro and in vivo laboratory techniques. Hands-on training that uses laboratory animals for skills building or increased understanding of complex processes has been shown to be beneficial to students in a variety of fields. Therefore, they will work with the animals by inducing experimental inflammation in rats, performing oral treatments with plant extracts, separating serum, performing biochemical tests for the oxidative stress and inflammation evaluation: total oxidative status, total antioxidant capacity, oxidative stress index, nitric oxide, total thiols and malondialdehyde. In the end the students will perform statistical analysis of the results and discuss the results according to the literature review.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No. We will provide guidance and protocols once the student is in the lab. The student is encouraged to read about inflammation and oxidative stress in order to build his/her general background knowledge.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
What skills are required of the student? The students will have to make intramuscular injections in rats, but no previous skills are required. Subjects passed: biochemistry, biology
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- Naima Saeed ; Muhammad R Khan and Maria Shabbir. ANTIOXIDANT ACTIVITY; TOTAL PHENOLIC AND TOTAL FLAVONOID CONTENTS OF WHOLE PLANT EXTRACTS TORILIS LEPTOPHYLLA L. . BMC Complementary and Alternative Medicine 2012 12:221; doi:10.1186/1472-6882-12-221
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