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(Cluj-Napoca) Use of natural plants extracts in preventing oxidative stress and chronic inflammation in different pathologic conditions
Romania (FASMR) - University of Medicine and Pharmacy "Iuliu Hatieganu", Cluj-Napoca
Pharmacology, Toxicology and Clinical Pharmacology
Ioana Corina Bocsan
Ioana Corina Bocsan, Raluca Pop
Type of Research Project
- Basic science
What is the background of the project?
Use of natural plants extracts in preventing oxidative stress and chronic inflammation in different pathologic conditions
What is the aim of the project?
The aim of the research is to test the influence of plant extracts on inflammation and oxidative stress parameters, using and experimental model.
What techniques and methods are used?
The student contributes to design the experimental protocol, followed by the preparation of the substances that will be tested. The next step is to do the experimental model of inflammation and they will test the influence of plants extracts on inflammation degree. The student will analyse the samples in order to determine oxidative stress and inflammatory parameters and afterwards analyse the obtained data.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student should do first a short review of literature in order to determine the most suitable experimental protocol. He/she will contribute together with the supervisor to design the experimental protocol. The student will observe how we do the randomization of animals, how we induce the inflammation and what kind of tests are suitable to analyse the inflammation degree. At different time points, according to the protocol, the student will observe how we perform the inflammation tests and how we take blood samples. After that the students will do Enzyme-linked Immunosorbent Assay (ELISA) tests into the lab in order to determine inflammatory and oxidative stress parameters. The student will analyse after that the raw data to obtain the final values.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, theoretical teaching will be provided by the tutors, as preliminary readings and discussions on the protocols.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student should know basic rules in the laboratory, how to use a pipette and how to manipulate experimental animals.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- Boarescu PM; Boarescu I; Bocșan IC; Pop RM; Gheban D; Bulboacă AE; Nicula C; Râjnoveanu RM; Bolboacă SD. CURCUMIN NANOPARTICLES PROTECT AGAINST ISOPROTERENOL INDUCED MYOCARDIAL INFARCTION BY ALLEVIATING MYOCARDIAL TISSUE OXIDATIVE STRESS; ELECTROCARDIOGRAM AND BIOLOGICAL CHANGES. 2019 Aug 1;24(15).
- Boarescu PM; Boarescu I; Bocșan IC; Pop RM; Gheban D; Bulboacă AE; Dogaru G; Bulboaca SD. EXPERIMENTAL MODEL OF ACUTE MYOCARDIAL INFARCTION EVALUATION OF PREVENTION AND REHABILITATION STRATEGIES IN CARDIOVASCULAR DISEASES - A PILOT STUDY. Balneo Research Journal 2019;10(3):288-293
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