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Study of IL-33/ST2 pathway in immuno-checkpoint blockade therapy against cancer.
China (IFMSA-China) - Soochow University, Suzhou
Department of Immunology
Type of Research Project
- Basic science
What is the background of the project?
Introducing the right inflammation to the tumor microenvironment by expressing appropriate cytokines, such as IL-33 (interleukin-33), should help further improvement of the aggregation, activation and function of tumor infiltrating T lymphocytes and it might be an effective strategy for tumor immunotherapy. This study will provide insights of potential applications in tumor immunotherapy, that is cytokine-mediated immune therapy of cancer should provide new opportunities for enhancing the immune “checkpoint”-based approach.
What is the aim of the project?
Our study aims to further provide a new theory and mechanism of anti-tumor immune response mediated by IL-33/ST2.
What techniques and methods are used?
1) Setting up the cell culture of melanoma cells; 2) Induction of differentiation of purified T cells in vitro, using cytokines; 3) Setting up tumor-bearing mice model by intradermal injection of tumor cells; 4) Monitoring the tumor growth size of tumor-bearing mice; Analysis of the tumor microenvironment by flow cytometry, real-time PCR (Polymerase Chain Reaction) and immunofluorescence
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
1. Intradermal injection of tumor cells to the mouse; 2. Analyzing the infiltration and function of T lymphocytes and other immune cells through immunofluorescence and software ImageJ; 3. Conducting real-time PCR (Polymerase Chain Reaction) to detect the expression of cytokines and transcription factors will be detected; Observing the immunofluorescence will used to observe the infiltration of lymphocytes for frozen section of tumor tissue.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary readings will be provided by the tutor. In addition, the tutor will discuss and explain in detail about the project that students will be conducting. Students are encouraged to take part in the lab meeting, journal club and seminars.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
1. Cell culture of Melanoma cells; 2. RNA (Ribonucleic Acid) extraction using TRIzol™ Reagent; 3. Data analysis through Microsoft Excel.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- Gao X; Wang X; Yang Q; Zhao X; Wen W; Li G; Lu J; Qin W; Qi Y; Xie F; Jiang J; Wu C; Zhang X; Chen X; Turnquist H; Zhu Y*; Lu B*. Tumoral Expression of IL-33 Inhibits Tumor Growth and Modifies the Tumor Microenvironment through CD8+ T and NK Cells. J Immunol. 2015 Jan 1;194(1):438-45
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