Radiation effects of radionuclides in the effluent on sensitive aquatic animals- A zebra fish experiment
China (IFMSA-China) - Soochow University, Suzhou
State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection
Yu Tu;Fengmei Cui
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes No No No No
Type of Research Project
- Basic science
What is the background of the project?
The research on the radiological protection of non-human species has not progressed very well at home and abroad. In order to make better understand of ionizing radiation and to protect the ecological environment, it is urgent and necessary to carry out the research on radiation effects of non-human species.Notably, aquatic animals are typical of non-human species involved in the vicinity of nuclear power plants.This has lead to a research for the survival and reproduction change of related species. The zebrafish is gaining importance as a popular vertebrate model organism and is widely employed in ecotoxicological studies, especially for the biomonitoring of pollution in water bodies. So, the zebra fish will be used to dect the radionuclide toxicity.
What is the aim of the project?
Study on the toxicity of radionuclides in aquatic animals,especially for model animal zebra fish.
What techniques and methods are used?
echniques: Adult zebrafish were maintained separately as stock in aquaria (fitted with aerators and heaters) at 28±1 °C with 14:10 h (light: dark) photoperiods. Fishes were fed twice daily with live brine shrimps and commercial fish feed. As and when the eggs were needed for studies, 2 male and 2 females were placed in a hatching box in the aquaria in the late evening and allowed to breed for overnight. Spawning process was triggered in the early morning hrs of the day by switching on the lights which lasted around one h. After 3H exposure, mortality, hatching rate, malformations and body length of the larvae were employed as the toxicological endpoints at the individual level. Further, expression of genes associated with the development and DNA damage were evaluated using Quantitative PCR (qPCR). Analysis of the data obtained was carried out using the statistical package GraphPad Prism 5.0. A level of probability of p<0.05 was considered as a statistically significant data. Methods: Fish maintenance and egg production, fluorescence microscope observation, qPCR, ROS detection, etc.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The zebrafish were poisoned by 3 H. Samples were collected at different times to detect oxidative stress : reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT),Peroxidase (POD), glutathione detoxification (GST), malondialdehyde (MDA) content. The reproductive toxicity of zebra fish was observed. Different developmental toxicity endpoints were investigated. Further, expression of genes associated with the development and DNA damage were evaluated using Quantitative PCR (qPCR) Additionally,several data analysis may be required.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
More two lectures or seminars about radiation protection and expermintal skills are provided. Assoicate professor Fengmei Cui will give the lectures or seiminars.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
- The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No; None
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Praveen Kumar MK; Shyama SK; Kashif S; Dubey SK; Avelyno D; Sonaye BH; Kadam Samit B; Chaubey RC. Effects of gamma radiation on the early developmental stages of Zebrafish (Danio rerio). Ecotoxicol Environ Saf. 2017 Aug;142:95-101. doi: 10.1016/j.ecoenv.2017.03.054.