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Connecting inflammation, neuroscience and pharmacology
Federal University of Minas Gerais (UFMG)
Antonio Carlos Pinheiro de Oliveira
Antonio Carlos Pinheiro de Oliveira, Helton José dos Reis
Type of Research Project
- Basic science
What is the background of the project?
Neurodegenerative diseases are complex conditions to which there is no cure. Thus, it is important to evaluate the physiopathology and to develop treatments in order to stop their development. In the Laboratory of Neuropharmacology of the Federal University of Minas Gerais, we evaluate whether inflammation and different sigalling pathways are involved in neurodegeneration. We also investigate potential drugs for the treatment of these conditions. All the experiments are performed in animal models of Alzheimer’s disease, seizures and others.
What is the aim of the project?
Our project aim to inverstigate the role of inflammation in neurodegenerative diseases and psychiatric disorders.
What techniques and methods are used?
Animal behavior, immunohistochemistry, immunofluorescence, PCR, Western blot. To study the animal behavior we mainly observe physiological parameters. Therefore, food and water intake can be measured in mice, as well as the body weight and temperature. A rectal probe can be used to measure body temperature. To standardize stress effects, the mice can be pre-adapted to the measurement of rectal temperature for two days prior to the experiment. To analyze the production of cytokines through immunohistochemistry, plasma and brain extracts from mice are used and the essay is performed by an ELISA technique kit as described by the manufacturer. To analyze celular activation in brain sections, we can use immunofluorescence by the protocol previously described by Oliveira-Lima et al. (2015). Brain sections (free-floating) are incubated with primary antibodies specific the antigen. After that, we washe the sections and stain with species-specific secondary antibodies conjugated to Alexa Fluor 488 (Invitrogen). Sections are rinsed, mounted with Vectashield (Vector Labs) and examined on a Leica TCS confocal microscope (Leica Microsystems). PCR and Western blot techniques are also performed according to a protocol.
What is the role of the student?
- The student will mainly observe
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
To learn the tecniques in the field of neuroscience and to help undergraduated students in their experiments, such as manipulation of samples, learning how to use many useful laboratory equipaments and to develope a scientific line of work. We require the student to study the recommended articles. Besides, we expect the undergraduated student to present by the end of his research intership his experiences, outcomes and possible suggestions to improve the program.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No, but the student can participate in the weekly seminars of the laboratory. In the seminars we discuss about the currently developing researchs by our team. The seminars are lead by a professor or other laboratory researcher.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- OLIVEIRA-LIMA OC; CARVALHO-TAVARES J; RODRIGUES MF; GOMEZ MV; OLIVEIRA ACP; RESENDE RR; GOMEZ RS; VAZ BG; P MCX. Lipid dynamics in LPS-induced neuroinflammation by DESI-MS imaging. BRAIN BEHAVIOR AND IMMUNITY; v. 79; p. 186-194; 2019.
- OLIVEIRA BCL; BELLOZI PMQ; REIS HJ; OLIVEIRA ACP. Inflammation as a possible link between dyslipidemia and Alzheimers disease. NEUROSCIENCE; v. 376; p. 127-141; 2018.
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