Projects
Name
Sperm quality distribution in men attending the Fertility Center
University
Vilnius University
Domain
Reproductive Medicine
Departement
Department of Fertility Technologies and Gamete Bank, Vilnius University Hospital Santaros Klinikos
Head
Dr. Zivile Gudleviciene, senior clinical embryologist, MD, PhD
Tutor
Dr. Zivile Gudleviciene, senior clinical embryologist, MD, PhD
Languages
English (required)
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes No Yes No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Human infertility is a great problem today in the modern world. More and more couples suffer from infertility or subfertility every year. Infertility divides to women (35%), men (35%), due to both partners (20%) and unknown infertility (10%). The recent data shows that men sperm quality staying more and more abnormal. Frequency of male infertility is growing. It seems because of using computers, cell phones, exercising less and living more passive life style.
What is the aim of the project?
The aim of this project – to evaluate the quality of sperm in men attending to VUHSK and calculate the distribution of normal and abnormal sperm tests.
What techniques and methods are used?
Every male patient attending VUHSK Sperm Bank are taking part in the study. A sample is being taken from the sperm donor. Firstly, the specimen container is put in an incubator for liquefaction. After 30-60 minutes sperm appearance, viscosity is being assessed, volume, pH are being measured. Secondly, sperm smear is prepared for assessing microscopic appearance, sperm motility, vitality and the dilution required for assessing sperm number. Makler-Chamber camera is used for calculating sperm cells. Eosin-nigrin test is performed to determine sperm vitality. Before fixing and staining sperm smear, biochemical reactions could be performed if needed. Mixed antiglobulin reaction, peroxidase and immunobead tests could be performed. Stained samples are analyzed for sperm morphology. 200 sparmatozoa per replicate are assessed for determining the percentage of normal and abnormal forms. Abnormal spermatozoa are evaluated though microscope and classified according to their head defects (tapered, pyriform, round, amorphous, vacuolated, small acrosomal area), neck and midpiece defects (bent neck, asymmetrical, thick insertion, thin), tail defects (short, bent, coiled) and spermatozoa with excess residual cytoplasm are also featured. Finally, statistical descriptive analysis is performed using SPSS, which helps to evaluate overall sperm quality.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Students will know basic principles of infertile couple (focusing mainly on men) examination, sperm test examination and evaluation. After this project student will be able to perform standard procedures such as the sperm test by himself, make diagnosis and explain to men the main parameters of sperm test. Statistical analysis will be performed by the student using SPSS. During the project, students will learn how in vitro fertilization is performed, the student will have a chance to observe various other procedures performed at the Fertility Center — ovarian punctions, embryo transfers, embryo freezing and other procedures, which are done before and after in vitro fertilization. Finally, based on collected data, a presentation will be prepared by the student.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, preliminary reading and seminars during the practical job.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- The student will prepare a presentation
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge in biomedical science and laboratory procedures. We also expect that the student will attend at least 80% of the research exchange, be responsible for his/her actions at the practice and be accountable to the supervisor and LORE/NORE.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Students in biomedical fields
Articles
- World Health Organization. (2010). WHO laboratory manual for the examination and processing of human semen. Geneva: World Health Organization
- Barratt; C.L.R.; Björndahl; L.; De Jonge; C.J.; Lamb; D.J.; Osorio Martini; F.; McLachlan; R.; Oates; R.D.; van der Poel; S.; St John; B.; Sigman; M.; Sokol; R. and Tournaye; H. (2017). The diagnosis of male infertility: an analysis of the evidence to support the development of global WHO guidance—challenges and future research opportunities
- Levine; H.; Jørgensen; N.; Martino-Andrade; A.; Mendiola; J.; Weksler-Derri; D.; Mindlis; I.; Pinotti; R. and Swan; S.H. (2017). Temporal trends in sperm count: a systematic review and meta-regression analysis