Projects
Name
Assessment of Dendritic cells functions in HCV infected patients on hemodialysis (Beni Sweif University)
University
Egypt (IFMSA Egypt) - Beni Sweif University, Beni Sweif city
Domain
Biochemistry
Departement
Biochemistry
Head
Dr. Ahmed Reda
Tutor
Dr. Ahmed Reda
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes No No No No No No No No No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Over 200 million people worldwide are chronically infected with hepatitis C virus (HCV) and about a quarter of these will go on to develop cirrhosis of the liver (María et al., 2013). Well-known risk factors for HCV transmission include injection drug use, blood product transfusion, organ transplantation, chronic hemodialysis (HD), occupational exposure among health care workers, unprotected sexual contact and vertical transmission (Yen et al., 2003 ; Strader et al., 2004). Liver disease is a significant cause of morbidity and mortality in patients with end-stage renal disease (ESRD) treated by dialysis or transplantation and hepatitis C is the most common liver disease in renal dialysis patients (Fabrizi et al., 2002). Prevalence of anti-HCV antibody among HD patients is consistently higher than in general population indicating increased risk of acquiring HCV infection among HD patients. The reported incidence varies from country to country and depends upon type of assay used and execution trends for HD (Jasuja et al., 2009). Dendritic cells (DCs), originally described by Steinman and Cohn 1973 serve as a crucial link between innate and adaptive immunity (Banchereau and Steinman 1998).
What is the aim of the project?
The present study aimed to assess the dendritic cells functions in HCV infected patients on hemodialysis via analysis of co-stimulatory markers (CD83, CD86 and CD40), a co-inhibitory marker (PD-L1) in blood mononuclear cells and also to assess serum hyaluronic acid (as an accurate noninvasive marker in predicting significant fibrosis) and IL-10 levels to correlate their levels with dendritic cells markers.
What techniques and methods are used?
1- Patient selection with inclusion and exclusion criteria 2- The following laboratory investigations were done for all patients: Mononuclear cells were isolated from Peripheral blood by ficoll. Serum IL-10 was assayed by ELISA. Hyaluronic acid as a fibrogenic marker (an alternative to liver biopsy). The analytic procedure: Quantitave RT- PCR gene expression of co-stimulatory markers (CD83, CD86, and CD40), co-inhibitory marker (PD-L1) were evaluated in HCV-infected patients as well as for control subjects. Preparation of the reaction master mix for RT-PCR. Calculation of Relative Quantification (RQ) (relative expression). Estimation of Serum Hyaluronic Acid (HA). Estimation of Serum IL-10. Assay Procedure: First step: standard, samples and blank + biotinylated. Second step: streptavidin -HRP. Third step: TMB substrate. Fourth step: stop reaction. Fifth step: Reading and calculation.
What is the role of the student?
- The student will mainly observe
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
1. Assist in the laboratory investigations done for the patients. 2. monitor and observe the analytic procedure steps, taking notes on each steps. 3. Assist in the procedures done through the analytical process. 4. It is expected that the search for modern publications on this topic in peer-reviewed journals will be carried out by student on his/her own.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There will be a preliminary lectures and seminars by university lecturers and assistant lecturers at the beginning of the training and at the end.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The knowledge of the initial or basic level of molecular biology, biochemistry, internal medicine.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
Articles
- Abdel-Aziz F; Habib M; Mohamed M K (2000): Hepatitis C virus (HCV) infection in a community in the Nile Delta: Population description and HCV prevalence. Hepatology; 32:111-5. Afonina I; Zivarts M; Kutyavin I; Lukhtanov E; Gamper H; Meyer RB (1997): Efficient priming of PCR with short oligonucleotides conjugated to a minor groove binder". Nucleic Acids Res.25 (13):2657-60. Agrawal S; Gollapudi P; Elahimehr R; Pahl MV; Vaziri ND (2010): Effects of end-stage renal disease and haemodialysis on dendritic cell subsets and basal and LPS-stimulated cytokine production. Nephrol Dial Transplant. Mar; 25(3):737-46. Alexiewicz JM; Smogorzewski M; Fadda GZ; Massry SG (1991): Impaired phagocytosis in dialysis patients: studies on mechanisms. Am J Nephrol.;11:102–111. Allon M; Depner TA; Radeva M; Bailey J; Beddhu S; Butterly D; Coyne DW; Gassman JJ; Kaufman AM; Kaysen GA; Lewis JA; Schwab SJ(2003): Impact of dialysis dose and membrane on infection-related hospitalization and death: Results of the HEMO Study. J Am Soc Nephrol 14: 1863–1870. Allon M; Radeva M; Bailey J; Beddhu S; Butterly D; Coyne DW; Depner TA; Gassman JJ; Kaufman AM; Kaysen GA; Lewis JA; Schwab SJ (2005): The spectrum of infection-related morbidity in hospitalized haemodialysis patients. Nephrol Dial Transplant 20: 1180–1186. Al-Wakeel J; Malik GH; al-Mohaya S; Mitwalli A; baroudi F; el-Gamal H; Kechrid M (1996): Liver disease in dialysis patients with antibodies to hepatitis C virus.Nephrol Dial Transplant; 11: 2265-8. Ambuhl PM; Binswanger U; Renner EL (2000). Epidemiology of chronic hepatitis B and C among dialysis patients in Switzerland. Schweiz Med Wochenscber; 130: 341-8.
- Afonina I; Zivarts M; Kutyavin I; Lukhtanov E; Gamper H; Meyer RB (1997): Efficient priming of PCR with short oligonucleotides conjugated to a minor groove binder". Nucleic Acids Res.25 (13):2657-60.
- Agrawal S; Gollapudi P; Elahimehr R; Pahl MV; Vaziri ND (2010): Effects of end-stage renal disease and haemodialysis on dendritic cell subsets and basal and LPS-stimulated cytokine production. Nephrol Dial Transplant. Mar; 25(3):737-46.
- Alexiewicz JM; Smogorzewski M; Fadda GZ; Massry SG (1991): Impaired phagocytosis in dialysis patients: studies on mechanisms. Am J Nephrol.;11:102–111.
- Allon M; Depner TA; Radeva M; Bailey J; Beddhu S; Butterly D; Coyne DW; Gassman JJ; Kaufman AM; Kaysen GA; Lewis JA; Schwab SJ(2003): Impact of dialysis dose and membrane on infection-related hospitalization and death: Results of the HEMO Study. J Am Soc Nephrol 14: 1863–1870.
- Allon M; Radeva M; Bailey J; Beddhu S; Butterly D; Coyne DW; Depner TA; Gassman JJ; Kaufman AM; Kaysen GA; Lewis JA; Schwab SJ (2005): The spectrum of infection-related morbidity in hospitalized haemodialysis patients. Nephrol Dial Transplant 20: 1180–1186.
- Al-Wakeel J; Malik GH; al-Mohaya S; Mitwalli A; baroudi F; el-Gamal H; Kechrid M (1996): Liver disease in dialysis patients with antibodies to hepatitis C virus.Nephrol Dial Transplant; 11: 2265-8.
- Ambuhl PM; Binswanger U; Renner EL (2000). Epidemiology of chronic hepatitis B and C among dialysis patients in Switzerland. Schweiz Med Wochenscber; 130: 341-8.