Evaluating the Effects of Intravenous Lipid Emulsion on Some Lipophilic Drugs Toxicity in Adult Male Albino Rats (Beni Sweif University)
Egypt (IFMSA Egypt) - Beni Sweif University, Beni Sweif city
Forensic Medicine
Forensic Medicine
Prof.Ahmed Gamal
Prof.Ahmed Gamal
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No Yes No No No Yes Yes No No No No
Type of Research Project
- Basic science
What is the background of the project?
Intravenous lipid emulsion (ILE) is composed of triglycerides and phospholipids emulsifier. It provides calories and essential fatty acids within total parenteral nutrition (TPN) (Bania, et al., 2007). As local anesthetics are commonly used in the emergency department, overdoses can lead to disastrous complications including cardiac toxicity and arrest (Tierney, et al., 2016). So that, ILE has been reported to reverse cardiovascular collapse in overdoses of local anesthetic agents in addition to other medications. Over recent years, the use of lipid emulsions for the treatment of severe cardio toxicity has spread from bupivacaine to other cardio toxins, including antipsychotic agents and antidepressants (Jamaty, et al., 2010). Poisoning is one of the leading causes of mortality and morbidity in many countries. In 1955, poisoning was the most common cause of death in patients aged between 35 and 44 years. Tramadol was first manufactured in Germany in 1970 to relieve post-surgical and chronic pains (Ahmadi, et al., 2011). Tramadol is a synthetic opioid analgesic commonly prescribed for moderate to severe pain, usual doses being up to 200 mg/day (McKeon, et al., 2011).
What is the aim of the project?
• To assess the effects of ILE in treatment of Tramadol and Haloperidol toxicity on different biochemical and pathological parameters of liver, kidney and brain of the albino rat.
What techniques and methods are used?
This study was done to assess the effects of intravenous lipid emulsion in treatment of Tramadol and Haloperidol toxicity on different biochemical (alanine transaminase, Aspartate aminotransferase, Glucose, Albumin, Urea, Creatinine and creatine phosphokinase.) and pathological parameters of liver, kidney and brain in adult male albino rats. The study was carried out on 180 adult male albino rats weighting 150-200g. The duration of the study was two days. Animals were weighed and randomly allocated into six groups (30 rats each)
What is the role of the student?
- The student will mainly observe
What are the tasks expected to be accomplished by the student?
1.Monitor and write down the Effect of different doses of Intravenous lipid emulsion (ILE) on liver and kidney Function Tests and CPK in: - Tramadol 200 mg group - Tramadol 250 mg group 2. Monitor and write down the Effect of different doses of Intravenous lipid emulsion (ILE) on liver and kidney Function Tests and CPK in Haloperidol 2.6 mg group Yes, there'll be Analytical Data Analysis Performed by the: Author No Specific Software will be used The Student will also: 1. Monitor and observe the analytic procedure steps, taking notes on each steps. 2. Assist in the procedures done through the analytical process.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There will be a preliminary lectures and seminars Who will perform them? – The Tutor How often would they happen? – Once / Week
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- The student will prepare a presentation
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The knowledge of the initial or basic level of molecular biology, biochemistry, Forensic & Toxicology.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Abdel-Zaher; A.O.; Abdel-Rahman; M.S. and ELwasei; F.M. (2011): Protective effect of Nigella sativa oil against Tramadol-induced tolerance and dependence in mice: Role of nitric oxide and oxidative stress. NeuroToxicology; 32: 725-733. Abou El Fatoh; M. F.; Farag; M. R.; Sayed; S.A.E; Kamel M.A; Abdel- Hamid; N.E.; Hussein; M. A. and Salem; G.A. (2014): "Some Biochemical; Neurochemical; Pharmaco toxicological and Histopathological Alterations Induced by Long-term Administration of Tramadol in Male Rats International Journal of Pharma Sciences Vol. 4; No. 3: 565-571. Afshari; R. and Ghooshkhanehee; H. (2009): Tramadol overdose induced seizure; dramatic rise of CPK and acute renal failure. J.P.M.A.; 59(3):178. Ahmadi; H.; Hosseini; J. and Rezaei; M. (2011): Epidemiology of tramadol overdose in Imam Khomeini hospital; Kermanshah; Iran. Journal of Kermansha University of Medical Sciences; 15(1):72-7. Ahmadi; J. (2015): Tramadol Dependency Treatment: A New Approach. Journal of Addiction Medicine and Therapeutic Science; 2(1): 1-3. American Psychiatric Association (1997): Practice guidelines for the treatment of patients with schizophrenia; Am J Psych.; 154. Anderson; M. (2017): Poisoning with illicit drugs. Pediatrics and Child Health; 27 (9): 401-405. Andrezina; R.; Josiassen; RC.; Marcus; RN.; Oren; D.A.; Manos; G.; Stock; E.; Carson; WH. and Iwamoto; T. (2006): Intramuscular aripiprazole for the treatment of acute agitation in patients with schizophrenia or schizoaffective References 130 disorder: a double-blind; placebo-controlled comparison with intramuscular haloperidol. Psychopharmacology (Berl). 188(3): 281-92. Ardakani; Y.H. and Rouini; M.R. (2007): Pharmacokinetics of Tramadol and its three main metabolites in healthy male and female volunteers. Biopharmaceutics & Drug Disposition; 28: 526-533. Ardakani; Y.H. and Rouini; M.R. (2009): Pharmacokinetic study of Tramadol and its three metabolites in plasma; saliva and urine. DARU Journal of Pharmaceutical Sciences; 17(4): 245-255. Arslan; E.D.; Demir; A.; Yilmaz; F.; Kavalci; C.; Karakilic; E. and Çelikel; E. (2013): Treatment of Quetiapine Overdose with Intravenous Lipid Emulsion. (doi: 10.2302/kjm.2012-0010-CR) ; Keio J Med 62 (2) : 53–57. Atici; S.; Cinel; I.; Cinel; L.; Doruk; N.; Eskandari; G. and et al. (2005): Liver and kidney toxicity in chronic use of opioids: an experimental long term treatment model. J Biosci 30: 245-252. Babalonis; S.; Lofwall; M.; Nuzzo; P.; Siegel; A. and Walsh; S. (2013): Abuse liability and reinforcing efficacy of oral tramadol in humans. Drug and Alcohol Dependence; 129: 116–124. Baldessarini; R.J. and Frankenburg; F.R. (1991): Clozapine. A novel antipsychotic agent. N Engl J Med; 11:746-754. Banchroft; J.; Stevens; A. and Turner; D. (1996): Theory and practice of histological techniques. 4th Ed. Churchil Livingstone; New York; London; San Francisco; Tokyo. Bania; T.C.; Chu; J.; Perez; E.; Su; M. and Hahn; I.H. (2007): Hemodynamic effects of intravenous fat emulsion in an animal model of sever verapamil References 131 toxicity resuscitated with atropine; calcium; and saline. Acad. Emerg. Med.; 14:105-111. Bania; T.C. (2011): Intravenous Fat Emulsion. In: Goldfrank’s Toxicologic Emergencies. By: Nelson; L.S.; Lewin N.A.; Howland; M.A.; Hoffman R.S.; Goldfrank; L.R.; Flomenbaum; N.E.; (eds.); 8th edition.; McGraw-Hill Professional; New York; 21: 976-981. Barar; F.S.K (2006): Essentials of pharmacotherapeutics. S Chand publication. Bartlett; D. (2014): Intravenous Lipids Antidotal Therapy for Drug Overdose and Toxic Effects of Local Anesthetics. Crit. Care. Nurse; 34(5):62-67. Beakley; B.M.; Kaye; A.D. and Kaye; A. (2015): Tramadol; Pharmacology; Side Effects; and Serotonin Syndrome: A Review. Pain Physician; 18: 395-400. Behl; C.; Lezoulac'h; F.; Widmann; M.; Rupprecht; R. and Hoslboer; F. (1996): Oxidative stress-resistant cells are protected against haloperidol toxicity. Brain Res.; 717(1-2):193-5. Biała; G. (2000): “Haloperidol-induced catalepsy is influenced by calcium channel antagonists;” Acta Poloniae Pharmaceutica; vol. 57; no. 3; pp. 233–237. Bloor; M.; Paech; M.J. and Kaye; R. (2012): Tramadol in pregnancy and lactation. International Journal of Obstetric Anesthesia; 21: 163-167. Brandish; E.; Owen; C. and Sinclai; J. (2016): Acute management of alcohol and other drug problems. Medicine; 45 (2): 68-73. Brancaccio; P.; Maffulli; N. and Limongelli; F. M. (2007): Creatine kinase monitoring in sport medicine. Br. Med. Bull.; 81(1):209- 230. References 132 Brockmoller; J.; Kirchheiner; J. and Schmider; J. (2002): the impact of the CYP2D6 polymorphism on haloperidol pharmacokinetics and on the outcome of haloperidol treatment. Clin Pharmacol Ther; 4:438-452. Bucklin; M.H.; Gorodetsky; R.M. and Wiegand; T.J. (2013): Prolonged lipemia and pancreatitis due to extended infusion of lipid emulsion in bupropion overdose. Clin. Toxicol. 51(9):896- 898. Campbell; D. (2001): The management of acute dystonic reactions. Austra Presc.;24:19-20. Carpentier; Y.A.; Richelle; M.; Bury; J.; Bihain; B.E.; Olivecrona; T. and Deckelbaum; R.J. (1987): Phospholipid excess of fat emulsion slows triglyceride removal and increases lipoprotein remodelling. Arteriosclerosis 7:541a; Carreiro; S.; Blum; J.; Jay; G. and Hack; J.B. (2013): Intravenous lipid emulsion alters the hemodynamic response to epinephrine in a rat model. J. Med. Toxicol.; 9: 220-225. Casey; D.E. (1994): Motor and mental aspects of acute extrapyramidal syndromes. Acta Psychiatr Scand ; 89(suppl 380):14-20. Castanares-Zapatero; D.; Wittebole; X.; Huberlant; V.; Morunglav; M. and Hantson; P. (2012): Lipid Emulsion as Rescue Therapy in Lamotrigine Overdose. J. Emer. Med.; 42(1): 48-51.