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Development of osteocalcin biosensor for prognosis of the bone metabolism
Turkey (TurkMSIC) - Ege University, Izmir
Ege University Faculty of Medicine Medical Biochemistry Department Universite Cd. 35100 Bornova-İzmir
Assoc. Prof. Dr. Ebru SEZER
PhD. Student Zihni Onur UYGUN
Type of Research Project
- Basic science
What is the background of the project?
Please, provide the background of the project (Provide information about the project; min. 10 lines, max. 25 lines) Biosensors are analytical devices, that combination of immobilizing a biorecognition receptor, enzyme, receptor, antibody, DNA(Deoxyribonucleic Acid) or protein molecule, on a physicochemical transducer, which shows specific affinity to the analyte molecule. The signals gained from the interaction between the biological recognition element on the transducer and the analyte molecule are transmitted to the analysis system by the 'transducer' and the measurement is carried out by analyzing the concentration-proportional response of the signal. Therefore, they have highly selective and sensitive potential for hand-held-usage. In this project, we use antiosteocalcin biomolecules as biorecognition receptors to bind osteocalcin which is used as a bone turnover biomarker to monitor osteoporosis. This biosensor is designed as a electrochemical affinity based biosensor system. The fundamental of the detection method is chronoimpedance, which is a time dependent resistance measurement system. Also in Turkey Turkish Biochemical Society established in 1975 .Its aim is to improve the science of biochemistry in our country and to improve biochemistry education at undergraduate and graduate level.
What is the aim of the project?
The aim of the project is to develop an osteocalcin biosensor as a bone turnover marker monitoring system for osteoporosis. It provides fast, sensitive and practice method for the detection of the molecule in blood with a low cost.
What techniques and methods are used?
What techniques and methods are used? Mention the steps/stages in this project. (Provide examples and avoid abbreviations; which tools will be used in general in this project?) In this project, highly conductive graphene based screen printed electrodes will be used as transducers. Graphene layers has an ability to form active carbonyl groups to bind molecules covalently, in this project those will be used as anti-osteocalcin antibodies. The , which is a technique to assess electrode-electrolyte interface monitoring. We will use this method to assess these molecules’ interactions. The interaction will be performed by chronoimpedimetrically, which means time dependent electrode surface resistance measurement. First of all, electrodes will be activated by acidic conditions and electrical conditions to form active carbonyl groups on the graphene electrodes, afterwards, these groups will be chemically modified by NHS(N-hydroxysuccinimide)/EDC(N-ethyl-N′-(3-(dimethylamino)propyl)carbodiimide)couple to bind anti-osteocalcin molecules. These molecules will be able to bind osteocalcin in serum and considered by their chronoimpedance. The proposed project is expecting the data, which is shown in figure 1 as schematic representation(Figure 1.).
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
What are the tasks expected to be accomplished by the student? (Minimum 10 lines, provide detailed explanation) The following tasks will be expected to be accomplished by the student: -Learning to using osteocalcin and anti- osteocalcin antibodies effects to organisms -Learning to bone turnover markers like; Markers that are specific to bone formation include bone-specific alkaline phosphatase (BSAP), osteocalcin, and N-terminal propeptide of type I procollagen (PINP); markers specific to bone resorption include N-terminal telopeptide of type I collagen (NTX), C-terminal telopeptide of type I collagen (CTX), and pyridinoline cross-links -Learning ossification types like ; endocondral, intramembranous ossification -Understanding why osteocalcin-binding molecules such as NHS(N-hydroxysuccinimide) triggers an electrochemical impedance on the electrodes. -Learning about graphen structure and using area for biological organisms.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Will there be any theoretical teaching provided for the student (preliminary readings, lectures, courses, seminars)? Please describe how much teaching and by whom. The following topics will be provided in the form of seminars by Assoc. Prof. Dr. Ebru SEZER. Lessons will be provided according to the availability of the teacher, at least once per week: -Laboratory protocols -Introduction to bone biochemistry -Measurement of the interaction between anti-osteocalcin and osteocalcin by electrochemical impedance spectroscopy
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No specific skills are required.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- 1. Ertugrul; Hilmiye Deniz; and Zihni Onur Uygun. 2016. “Impedimetric Biosensors for Label-Free and Enzymless Detection.” Intech i(tourism): 13. https://www.intechopen.com/books/advanced-biometric-technologies/liveness-detection-in-biometrics.
- 2. Sezginturk; Mustafa Kemal; and Zihni Onur Uygun. 2012. “An Impedimetric Vascular Endothelial Growth Factor Biosensor-Based PAMAM/Cysteamine-Modified Gold Electrode for Monitoring of Tumor Growth.” Analytical Biochemistry 423(2): 277–85. https://www.sciencedirect.com/science/article/pii/S0003269712000085 (August 1; 2019).
- 3. Uygun; Zihni Onur et al. 2018. “Fullerene-PAMAM(G5) Composite Modified Impedimetric Biosensor to Detect Fetuin-A in Real Blood Samples.” Analytical Biochemistry 542: 11–15. https://www.sciencedirect.com/science/article/pii/S0003269717304542 (August 1; 2019).
- 4. Uygun; Zihni Onur; and Mustafa Kemal Sezginturk. 2011. “Biosensors for Cancer Biomarkers.” Biosensors - Emerging Materials and Applications.
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