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Use of 3D Bioprinting for generation of artificial tissue and organs
Turkey (TurkMSIC) - Istinye University, Istanbul
Istinye University School of Medicine Histology & Embryology Dept. Edirne Cirpici Yolu No:9 , 34025 Cevizlibag / Istanbul / TURKEY
Assistant Professor Hakan DARICI, Ph.D.
Assistant Professor Hakan DARICI, Ph.D.
Type of Research Project
- Basic science
What is the background of the project?
Please, provide the background of the project (Provide information about the project; min. 10 lines, max. 25 lines) As Stem Cell and 3D Labs, we work jointly to perform various projects on different subjects. We do stem cell differentiation experiments to figure out stem cell limits. Also, we develop Artifical Intelligence to find out cell cycle mutation analyses. We design artificial tissues and organs to be used in high damaged burn accidents by 3D Bioprinting. Also, we can do artificial blood vessels, skin, cartilage and bone tissues with 3D Bioprinting. The prostheses used in limb amputations are far from natural and cause psychological trauma especially for children. We aim to make prostheses in the image closest to the original limb. We aim to do this by covering the prosthesis with artificial leather. Biosensor systems for the health sector are developed in our laboratories. These systems are intended to be used in the analysis of various biomarkers ( proteins, nucleic acids, toxic substances, etc.) In Turkey there are not many studies based on 3d bioprinting, with this study we hope to contribute the lack of the studies on 3d bioprinting. In our university’s Histology & Embryology Department there are currently 7 specialists working. In Turkey there is also a society called “Turkish Histology and Embriology Society” that was established in 1990. This society does various actions based on Histology and Embriology like organizing National and International congress.
What is the aim of the project?
Our ultimate aim is to generate artificial human tissues for replacement or enhancement of human organs or limbs. To reach that aim we work on several small projects to develop each required artificial tissue like bone, blood vessels, connective tissues and skin. We use state-of-the-art methods lice 3D bioprinting, robotics, microfluidics and AI analyses within these projects.
What techniques and methods are used?
What techniques and methods are used? Mention the steps/stages in this project. (Provide examples and avoid abbreviations; which tools will be used in general in this project?) Following protocols will be used in the various projects. Methods include; - Stem cell culture methods (passaging, freezing&thawing, cell counting) - Preparation of various chemical solutions to create specific effects (differentiation, proliferation, pathaway inhibition etc.) - Immunofluorescence staining of cultured cells using specific antibodies - Fluorescent microscope use for visualising stained cells - 3D design of objects via CAD softwares for artificial organ modelling - 3D printing of anatomical models and protheses from plastic filaments - Preparation of biocompatible and biodegradable hydrogels (bioinks) for 3D bioprinting - 3D Bioprinting of artificial tissues using bioprinters - Culture of 3D bioprintied tissues and observing cellular differentiation and organ formation - Coding of AI software using pyton - Analyses via AI software (Following of general safety rules and sterile techniques are expected from the applciants. Since most of the methods we use are highly specific and new, we teach them to the accepted students, again according to their interest.)
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
What are the tasks expected to be accomplished by the student?
Most important thing we expect from students is to follow the lab safety and sterile technique procedures so they not only harm themselves and environment, but also to noyt harm or contaminate other projects. They were also expected to help ongoing projects, according to their interest as lab assistants. The tasks start simple with laboratory clean-up and standart cell culture, and become complex like media preparation, simple 3D design, 3D printing and bioprinting, histochemical or immunofluorescent staining to most specific subjects like AI (Artifical Intelligence) software coding.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The students will be thought witch clinical seminars all necessary subjects prior to their lab work. However, they can gather info from outside sources like books, articles (listed below), YouTube education videos about cell culture, stem cells, 3D printing and bioprinting. They can also have info about AI coding (optional for students who want to participate AI studies). Teaching will be provided by Asst. Prof. Hakan DARICI at least once per week.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Students must have successfully passed 2st year medical lessons Previous experience with: Cell culture lab or 3D printing or Coding language (phyton) (Optional)
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
- 1) Pediatr Res. 2018 Jan;83(1-2):223-231. doi: 10.1038/pr.2017.252. Epub 2017 Nov 1.3D bioprinting using stem cells.Ong CS1; Yesantharao P1; Huang CY2; Mattson G1; Boktor J1; Fukunishi T1; Zhang H1; Hibino N1.
- 2) Adv Drug Deliv Rev. 2018 Jul;132:296-332. doi: 10.1016/j.addr.2018.07.004. Epub 2018 Jul 7.3D bioprinting of tissues and organs for regenerative medicine.Vijayavenkataraman S1; Yan WC2; Lu WF3; Wang CH2; Fuh JYH4.
- 3) Adv Healthc Mater. 2018 Jan;7(1). doi: 10.1002/adhm.201701018. Epub 2017 Nov 29. Organ Bioprinting: Are We There Yet? Gao G1;2; Huang Y1; Schilling AF3; Hubbell K2;4; Cui X1;2;4.
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