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Serotonergic modulation of the Ventral Cochlear Nucleus
Turkey (TurkMSIC) - Gaziantep University, Gaziantep
University of Gaziantep Faculty of Medicine, Departmant of Physiology, 15 Temmuz Mah.,Universite Cd., 27310 Sehitkamil / GAZIANTEP
Prof. Dr. Ramazan Bal
Prof. Dr. Ramazan Bal
Type of Research Project
- Basic science
What is the background of the project?
Please, provide the background of the project (Provide information about the project; min. 10 lines, max. 25 lines) Investigating how the electrical properties of neuron changes when exposed to specific agonist/antagonists of serotonin receptor subtypes of neuron types present in the ventral cochlear nucleus and whether their physiological activity during individual processing of acoustic signal is affected. There is a patch-clamp system in the electrophysiological laboratory and active studies are continuing. How the receptor affect cell excitability whether the action potential contributes to the shape and frequency, will be investigated by the current clamp technique. Then, channel current caused by serotonin receptor subtypes will be isolated using specific ion channel agonist-antagonist using the voltage clamping technique. Inactivation and deactivation kinetics and biophysical properties of these cochlear nuclear neurons will be introduced for the first time.
What is the aim of the project?
What is the aim of the project? (Highly specific: purpose?) The aim of this study is to determine the serotonin receptor subtypes of neuron types in the ventral cochlear nucleus.
What techniques and methods are used?
Electrophysiology is one of the foundational disciplines in neuroscience and cardiac physiology for the evaluation of ion channels. The Patch-clamp technique is a versatile electrophysiological tool for understanding ion channel behavior. Patch clamp technique is an up-to-date technique that allows the electrophysiological study of ion channels. The study will be carried out by patch clamping method in Mouse brain sections. After brain sectioning, once the animals decapitated under anesthesia, the head of the animal will be immersed in continuously oxygenated artificial brain fluid. Artificial cerebrospinal fluid (aCSF) is a biological buffer that enables a vital environment for neuronal tissue by maintaining the homeostasis, osmolarity, and pH at physiological levels. ACSF is commonly used for many lab experiments including in vitro and in vivo applications. The physiological oxygenated artificial brain fluid solution for 1000ml will contain 130 mM NaCl,3 mM KCl,1.2 KH2PO4 mM, 2.4 CaCl2 mM, 1.3 MgSO4 mM, 20 NaHCO3 mM, 3 HEPES (zwitterionic organic buffer),10 glucose. The electrophysiological data will be transferred to the computer via an analog/digital converter with Digidata and Clampex,the pClamp software for stimulation and data acquisition,and Clampfit (Axon Instruments) for analysis will be used.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
What are the tasks expected to be accomplished by the student? (Minimum 10 lines, provide detailed explanation) The following tasks will be expected to be accomplished by the student: -Learn to analyse the electrophysiological data transferred to computer with tutor -Observing the laboratory animal experiments on the mice which will be done by the tutor. -Examining the Patch Clamp technique steps and applications -Knowing the neurotransmitter receptor types in nerve cells -Learning mechanism of nerve cell stimulation -Learning the ethical code and guidelines for experimenting with animals. -Being able to use Statistical Package for the Social Sciences (SPSS) 26 and Microsoft Excel at a fundamental level for the evaluation of the project results.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Will there be any theoretical teaching provided for the student (preliminary readings, lectures, courses, seminars)? Please describe how much teaching and by whom. The following topics will be provided in the form of seminars by Prof. Dr. Ramazan Bal. Lessons will be provided according to the availability of the teacher, at least once per week: -General information about the Patch Clamp Technique -Explaining neurotransmitter receptor types in nerve cells -General information for analyses to the electrophysiological data use softwares : the pClamp software for stimulation and data acquisition,and Clampfit (Axon Instruments)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No specific skills are required.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Hear Res. 2017 Feb;344:13-23. doi: 10.1016/j.heares.2016.10.017. Epub 2016 Nov 9. Serotonin modulates response properties of neurons in the dorsal cochlear nucleus of the mouse. Felix RA 2nd1; Elde CJ2; Nevue AA2; Portfors CV2. Felix RA 2nd; Elde CJ; Nevue AA; Portfors CV
- Hear Res. 2017 Feb;344:13-23. doi: 10.1016/j.heares.2016.10.017. Epub 2016 Nov 9.Serotonin modulates response properties of neurons in the dorsal cochlear nucleus of the mouse. Felix RA 2nd1; Elde CJ2; Nevue AA2; Portfors CV2.
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