Development of preclinical models for colon cancer
Austria (AMSA) - University of Vienna, Vienna
Department of Pathology
Univ.-Prof. Dr. Renate Kain, PhD
Loan Tran, Thomas Dillinger
English, German
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Basic science
What is the background of the project?
Targeted therapies and progress in molecular pathology have been an important step towards precision medicine in cancer. Colorectal cancer is among the most frequent cancers in men and women in Western countries and targeted therapies are already applied in advanced stages of disease. However, due to the occurence of drug resistance, these therapies have shown limited success. The newly developed 3D cell culture system known as “organoid culture” allows long-term expansion of stem cells and tumor cells into self-organizing, multicellular and functional structures with properties resembling those of the original tissues. These tumoroids are genetically amenable and conserve (epi)genetic alterations as well as histopathological features without the contamination of other stromal cell types and are therefore perfectly suited for high-purity NGS profiling, disease modeling or drug screening approaches.
What is the aim of the project?
The aim is to generate a living biobank of normal and malignant specimen from patients with colorectal cancer undergoing surgery, as well, these organoid cultures will be characterized on molecular and histo- pathological levels and used for functional studies and drug testing.
What techniques and methods are used?
Advanced cell culture, next generation sequencing technologies, immunohistochemistry, molecular pathology, drug testing. Organoid cultures: Healthy tissue-derived organoids will be cultured in Human Intestinal Stem Cell medium (HISC). The composition of HISC is: Basal culture medium with 50% Wnt conditioned medium, 20% R-Spondin conditioned medium, 10% Noggin conditioned medium, 13 B27, 1,25 mM n-Acetyl Cysteine, 10 mM Nicotinamide, 50 ng/ml human EGF, 10 nM Gastrin, 500 nM A83-01, 3 uM SB202190, 10 nM Prostaglandin E2, and 100 mg/ml Primocin (Vivogen). Tumor organoids will be cultured in HICS minus Wnt. For detailed experimental procedures, see article below.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will work in a research setting and should have basic skills in molecular biology related techniques. The student will perform hands on experiments and analyses (tissue culture, immunohistochemistry, DNA isolation, Immunofluorescence staining), after obtaining enough qualification within the daily supervision by one of our tutors. The student will learn to interpret data and will be responsible for her/his own project. A protocol will be required after the completion of the internship.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student will obtain literature related to the project. The project will be discussed with the supervisor and the tutors. Hands on training will be provided for all practical experiments
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic molecular biology skills
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Students in biomedical fields