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Exosomes and extracellular vesicles in liver physiology and pathophysiology.
Spain (IFMSA-SPAIN)-University Complutense of Madrid, Madrid
HepatoGastro Lab. Edificio de Medicina y Cirugía Experimental del Hospital Gregorio Marañón. c/ Doctor Esquerdo 46. Madrid 28007.
Javier Vaquero Martín
Javier Vaquero Martín
Required: English, Accepted: Spanish
Type of Research Project
- Basic science
What is the background of the project?
Liver disease is one of the main health problems in the world, with a big socio-economic impact. In addition, the liver has a spectacular capacity of regeneration. The mechanisms of liver disease and its complications, and of liver regeneration, however, are still incompletely elucidated. Recent studies suggest that intercellular and interorgan communication via the release of exosomes and other extracellular vesicles (EVs) play a major role in the normal functioning of the liver and in liver-related diseases. Experimental animal models of liver disease and regeneration represent a valuable tool for assessing the role of EVs in these settings and for identifying new potential therapeutic approaches.
What is the aim of the project?
To determine the role of extracellular vesicles on liver disease and its complications and on liver regeneration.
What techniques and methods are used?
The project has sub-projects in which different experimental models of liver disease and regeneration are used (e.g. Mice: partial hepatectomy, acetaminophen-induced acute liver injury; Rats: partial portal vein ligation, carbon tetrachloride-induced cirrhosis, porto-caval anastomosis). Also, there is a wide range of techniques that are used in the projects (physiology/hemodynamic measurements, extraction of proteins and nucleic acids, analyses of mRNA (real-time RT-PCR) and protein (Western-blotting, immunohistochemistry, ELISAs) expression, flow cytometry, primary culture of mouse hepatocytes, cell lines, isolation of exosomes by ultracentrifugation, size-exclusion chromatography and others, confocal microscopy, enzymatic assays and others.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
- To get a scientific background on the research lines, including critical reading of manuscripts. - To familiarize with the methods and techniques employed. - To help with the necropsy and collection of samples in the experimental animal models, under supervision. -To acquire progressive independence in the performance of some of the techniques used in the experiments. - To familiarize and understand the ethics of research involving animals.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, he will be given some manuscripts and reviews related to the research line, and will attend any lab meetings and seminars that may be done during his/her stay.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Interest, responsibility, attention, initiative. Basic skills on literature search and laboratory work are a plus, but not an absolute requirement.
Are there any legal limitations in the student’s involvement
The student’s participation on experiments involving animals will have to be under close supervision by researcher accredited for animal research
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
- 1. Fortea JI; Fernandez-Mena C; Puerto M; Ripoll C; Almagro J; Bañares J; Bellón JM; Bañares R; Vaquero J. Comparison of two protocols of carbon tetrachloride-induced cirrhosis in rats – Improving yield and reproducibility. Sci Rep 2018; 8: 9163. IF:4;259. Q1.
- 2. Fortea JI; Zipprich A; Fernandez-Mena C; Puerto M; Bosoi CR; Almagro J; Hollenbach M; Bañares J; Rodríguez-Sánchez B; Cercenado E; Clément MA; Rose CF; Bañares R; Vaquero J; Ripoll C. Enoxaparin does not ameliorate liver fibrosis or portal hypertension in rats with advanced cirrhosis. Liver Int 2018; 38: 102-112; doi: 10.1111/liv.13510. IF: 4.47. Q1.
- 3. Asencio JM; García-Sabrido JL; López-Baena JA; Olmedilla L; Peligros I; Lozano P; Morales-Taboada Á; Fernández-Mena C; Steiner MA; Sola E; Perez-Peña JM; Herrero M; Laso J; Lisbona C; Bañares R; Casanova J; Vaquero J. Preconditioning by portal vein embolization modulates hepatic hemodynamics and improves liver function in pigs with extended hepatectomy. Surgery 2017; 161: 1489-1501. IF: 3.309 (in 2015) Q1.
- 4. Nehrhoff I; Bocancea D; Vaquero J; Vaquero JJ; Ripoll J; Desco M; Gómez-Gaviro MV. 3D imaging in CUBIC-cleared mouse heart tissue: going deeper. Biomed Opt Express 2016; 7: 3716-3720. IF: 3.344 (in 2015). Q1.
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