Projects
Name
Natural Killer Subsets in Patients with Fabry Disease
University
Turkey (TurkMSIC) - Meram University, Konya
Domain
Internal Medicine
Departement
Department of Internal Medicine, Necmettin Erbakan University, Faculty of Medicine, Hocacihan Mh., 42080 Konya, Turkey
Head
Prof. Dr. Kultigin TURKMEN
Tutor
Prof. Dr. Kultigin TURKMEN
Languages
Turkish, English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No No Yes No No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Please, provide the background of the project (Provide information about the project; min. 10 lines, max. 25 lines) Fabry Disease (FD) is a storage disorder which affects mostly renal, cardio and cerebrovascular systems. The lysosome, whose function is impaired in Fabry Disease is also an important compartment for the innate immune responses. To date, invariant natural killer (NK) T cell functions was found be impaired in Fabry Disease. Hovewer, there is no data regarding Natural Killer cell subsets in patients with Fabry Disease. We aimed to analyze subtypes of Natural Killer cell in patients with Fabry Disease-related chronic kidney disease (CKD) and proteinuria and compared these results those of with healthy subjects. The differences of NK subset distributions between the two groups will be analysed using Statistical Package for Social Sciences 26 (SPSS26), and we will look for a relationship between NK subset distribution and development of CKD and proteinuria in Fabry Disease.
What is the aim of the project?
The main aim of the project is to highlight the role of Natural Killer cell in the pathogenesis of proteinuria and chronic kidney disease, thus elucidating if new options for the diagnosis, managent and detection of the stage of FD-related renal complications is possible using cytological techniques involving NK subset distributions.
What techniques and methods are used?
The main techniques involved with this project are: - flow cytometric isolation of Natural Killer cells - anaylsis of NK subsets using immunohistochemical staining on cell counting microscopes - sequencing of subset-specific proteins with polymerase chain reaction (PCR) - Running the PCR produce on gel electrophoresis (‘’Western Blotting’’) to supplement the results of cell counting - Measuring the levels of cytokines associated with different subsets of NK’s in patients with Fabry Disease and healthy controls via Enzyme-Linked ImmunoSorbent Assay (ELISA). - Analysing the cytotoxic capacity of different NK subtypes isolated from FD patients by observing cellular degeneration in vitro after combining NK isolates with cell cultures. - Cataloguing all raw data with Microsoft Excel to prepare for biostatistical analysis, which will be performed by SPSS 26.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
What are the tasks expected to be accomplished by the student? (Minimum 10 lines, provide detailed explanation) The student will: - Examine the structure and subset distribution of Natural Killer cells under immumohistochemisry assisted microscopy. - Observe the procedures of ELISA testing and record the results on Excel under supervision. - Observe the procedures of flow cytometry and record on Excel the raw data regarding the physical and chemical properties of NK subsets analysed. - Process the raw data on Excel to be put into SPSS 26 under supervision. Using SPSS 26 is not mandatory. - Discuss with the tutor all the procedures and results daily at the end of the shift. The tutor may request the notes the student has written during the day for this.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
As a mentor, Prof Dr Kultigin Turkmen will give lecture regarding Fabry Disease and NK cell pathobiology daily. The flow cytometric method will also be provided by the technicians daily.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- The student will prepare a presentation
- The student will prepare a scientific report
- The student will prepare an abstract
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Knowing the flow cytometry technique is also preferred but not required. The student should know the pathobiology of NK cells and the clinical aspects of Fabry disease.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
Articles
- Fabry Disease Prevalence in Renal Replacement Therapy in Turkey. Yalın SF; Eren N; Sinangil A; Yilmaz VT; Tatar E; Ucar AR; Sevinc M; Can Ö; Gurkan A; Arik N; Alisir Ecder S; Uyar M; Yasar M; Gulcicek S; Mese M; Dheir H; Cakir U; Köksal Cevher Ş; Turkmen K; Guven B; Guven Taymez D; Erkalma Senates B; Ecder T; Kocak H; Uslu A; Demir E; Basturk T; Ogutmen MB; Kinalp C; Dursun B; Bicik Bahcebasi Z; Sipahi S; Dede F; Oruc M; Caliskan Y; Genc A; Yelken B; Altıparmak MR; Turkmen A; Seyahi N. Nephron. 2019;142(1):26-33. doi: 10.1159/000496620. Epub 2019 Feb 8.
- The Prevalence of Fabry Disease in Patients with Chronic Kidney Disease in Turkey: The TURKFAB Study. Turkmen K; Guclu A; Sahin G; Kocyigit I; Demirtas L; Erdur FM; Sengül E; Ozkan O; Emre H; Turgut F; Unal H; Karaman M; Acıkel C; Esen H; Balli E; Bıtırgen G; Tonbul HZ; Yılmaz MI; Ortiz A. Kidney Blood Press Res. 2016;41(6):1016-1024. doi: 10.1159/000452605. Epub 2016 Dec 23.
- I nnate and Adaptive Immune Response in Fabry Diseas. Mauhin W; Lidove O; Masat E; Mingozzi F; Mariampillai K; Ziza JM; Benveniste O. JIMD Rep. 2015;22:1-10. doi: 10.1007/8904_2014_371. Epub 2015 Feb 18.
- Enzyme replacement therapy partially prevents invariant Natural Killer T cell deficiency in the Fabry disease mouse model. Macedo MF; Quinta R; Pereira CS; Sa Miranda MC. Mol Genet Metab. 2012 May;106(1):83-91. doi: 10.1016/j.ymgme.2012.02.014. Epub 2012 Mar 1.