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Circular RNA Expression In patient With Acne (6th of October University)
Egypt (IFMSA-Egypt) - 6th of October University, 6th of October City
Randa Nady Mohammed
Randa Nady Mohammed
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Acne vulgaris is a chronic inflammatory disease of the pilosebaceous unit,chartarized by the seborrhea,formation of comedones,erythematous papules and less frequently by nodules, deep pustules, or pseudocysts.The primary and the padiogoomonic lesion of acne is rnicrocomedo. amicroscopic lesion invisible to the eye. which evoh•es commonJy into inflammatory or noninflammatory lesions. The formationof microcomedo requires complex interplay of altered follicular keratinization , hyper plasia of the sebacous gland, and overcolonization of sebecous glands with Propionibacterium acnes
What is the aim of the project?
The aim of the current study is to evaluate Circular RNA expression in patient with acne and to correlate its level with acne severity .
What techniques and methods are used?
I.- Full history taking : • Including personal, Acne history as well as history of other Each skin diseases or drug intake 2.- Full clinical examination: • Complete general examination. • Complete cutaneous examination to evaluate the severity of acne ,clinical diagnosis with severe acne(grade IV) ) was confinncd by at least two dennatologists according to the Pillsbury grading system 3. -Determination of Ribonuclic acid • Total Ribonuclic acid will be extracted from blood sample usmg Trizol (lnvitrogen, Carlsbad, CA, United Stated of America ) Ribonuclease treatment will be perfonned to digest all the linear RiBonuclic. Sample will be purified with Ribonuclic acid clean beads, and the retrieved RiBonuclic acid will be fragmented using divalent cations at an elevated temperature. Random hexamer-primer will be used to synthesize the first-strand c-Deoxyribonucleic acid . Fragments will be purified with AMPure beads and resolved in EB buffer for end repair and adding A at the 3' end. Y-adaptor will be added afterwards. Then the product is amplified to construct the c-Deoxyribonuclic acid library. Finally, the library will pass quality control was perfonned by Illumina HiSeq 4000 platfonn on a I 00 bp paired end run.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
-performing general and local examination -Collecting medical history -reviewing all current modalities useful to provide An objective assessment for acne including : •simple clinical evaluation •taking biopsies and examining them •various perfusion management techniques including as (vital dyes ,video angiography ,video microscopy,and laser droplet ) -Generally some varsities in the task may be present according to different diagnostic stiuation -study and explain the basics of acne and their types and classification
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Theoretical teaching about history, examination, clinical skills, interpreting lap results in form of lectures andclinical talks By the tutor and the head departments
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student will prepare an abstract
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic skills in history taking •basic clinical dermatology skills .
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Assaf; H. A.; Abdel-Maged; W. M.; Elsadel; B. E.; Hassan; M. H.; Adly; M.A.; & Ali. S. A. (2016) Survivin as a novel biomarker in the pathogenesis of acne vulgaris and its corrc.lation to insulin-like growth factor-I. Disease markers; 2016. .
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