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Organoid: Organ in a dish for study of host-parasite interaction
Thailand (IFMSA-Thailand) - Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok
Chairman Assoc. Prof. Anchalee Tungtrongchitr
Type of Research Project
- Basic science
What is the background of the project?
Parasitic diseases continue to be a major threat to global public health, particularly in aging people whose immunity have gradually been deteriorated. Lack of treatment and prevention exacerbates this problem and emphasize the necessity of drug and vaccine development. Historically, the in vitro cell culture systems are crucial for evaluating efficacy and toxicity of drugs and vaccines in pre-clinical phase prior to translate into clinical use in human subjects. However, a major barrier has been a lack of an in vitro culture platform which is capable of recapitulating the in vivo human organs. Recently, human organoid, a three-dimensionally, self-organized cluster of cells, has become useful models to study host-parasite interaction. Potential of this platform for testing efficacy and toxicity of drugs and therapeutic proteins being used in patients will be investigated. This technology will facilitate and strengthen pre-clinical research of parasitic diseases in Thailand.
What is the aim of the project?
To develop human liver organoid, a three-dimensionally, self-organized cluster of cells from human induced pluripotent stem cells and apply as models of study of malaria-parasite interaction.
What techniques and methods are used?
Step 1.Generation of human liver organoid from induced pluripotent stem cells Techniques: Human induced pluripotent stem cell culture, Cell differentiation in vitro, Liver organoid culture and Cell imaging Step 2. Characterization of human liver organoid Techniques: gene expression analysis, albumin synthesis using a confocal imaging with fluorescence-tagged antibody, glycogen and lipid storage examination Step 3. Intrahepatic lifer cycle of Plasmodium Technique: Cell culture, Malaria culture
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Step 1.Generation of human liver organoid from induced pluripotent stem cells Student will culture human induced pluripotent stem cell and generate liver organoid. Step 2. Characterization of human liver organoid To analyze gene expression, student will extract RNA, synthesize cDNA and perform PCR. For albumin, student will use a confocal microscope to detect albumin using sets of fluorescence-tagged antibody. Moreover, capability of liver organoid in glycogen and lipid storage will examined by student too.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes. Before starting lab work, instructor will give short lecture relating to lab work. Also, students will have chance to participate in seminar in the campus.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Third year or above medical student with basic Immunology and Microbiology knowledge as well as molecular biology techniques. For basic immunology, student should know principle of antigen-antibody interaction-based assay such as ELISA or Western blot. Student also should have a basic knowledge in microbiology, including bacterial growth, antibiotics and sterile techniques in cell culture. Lastly, knowledge in DNA and RNA is required in order to analyze gene expression as well as extraction of both from cells.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Pre-Medical students from the American-British system - Students in biomedical fields
- Vascularized and functional human liver from an iPSC-derived organ bud transplant. Takebe T; Sekine K; Enomura M; Koike H; Kimura M; Ogaeri T; Zhang RR; Ueno Y; Zheng YW; Koike N; Aoyama S; Adachi Y; Taniguchi H. Nature. 2013 Jul 25;499(7459):481-4. doi: 10.1038/nature12271
- A comprehensive model for assessment of liver stage therapies targeting Plasmodium vivax and Plasmodium falciparum. Roth A; Maher SP; Conway AJ; Ubalee R; Chaumeau V; Andolina C; Kaba SA; Vantaux A; Bakowski MA; Thomson-Luque R; Adapa SR; Singh N; Barnes SJ; Cooper CA; Rouillier M; McNamara CW; Mikolajczak SA; Sather N; Witkowski B; Campo B; Kappe SHI; Lanar DE; Nosten F; Davidson S; Jiang RHY; Kyle DE; Adams JH. Nat Commun. 2018 May 9;9(1):1837. doi: 10.1038/s41467-018-04221-9
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