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The regulatory mechanisms of RE1-silencing transcription in regulating keratinocyte proliferation/differentiation balance during skin regeneration.
China (IFMSA-China) - China Medical University, Shenyang
Department of Stem Cells and Regenerative Medicine
Type of Research Project
- Basic science
What is the background of the project?
The deformity and dysfunction of skin caused by burn and trauma has become a common disease that endangers people's health. There are millions of skin trauma patients caused by burns and traffic accidents every year. The speed and quality of skin regeneration are directly related to the quality of life and social return of patients. The homeostasis of human skin epidermis depends on the balance between proliferation and differentiation of keratinocytes in the basement and upper basement. After skin injury, keratinocytes at the edge of injury accelerate proliferation and reconstruct the epidermis to cover the wound. The proliferation of keratinocytes is the main cell source for the reconstruction of epidermal structure, while the skin barrier function is formed by the differentiation of keratinocytes. The balance between proliferation and differentiation must be strictly and accurately regulated to maintain steady state and regeneration. We are interested in the regulating of biological behavior and function of skin keratinocytes during skin homeostasis maintenance and wound repair.
What is the aim of the project?
To determine the role of :RE1-silencing transcription factor ( REST )in regulating the proliferation/differentiation balance of keratinocytes and its significance for wound healing.
What techniques and methods are used?
1. Primary human skin keratinocyte will be isolated and cultured in this project. The cell line HaCaT (Human immortalized keratinocytes) will also be used in the experiments. The transfection of siRNA and vectors will be carried out using lipo 2000 regent. 2. The cells will be transfected and then real-time PCR (Polymerase chain reaction) will be applied to determine the mRNA levels of the related genes. Western blotting will be used to detect the protein levels. The location of the proteins in cells will be analyzed using immunofluorescent methods. 3. Data analysis and quantification data will be analyzed with SPSS (statistical software system).
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
1. Learn the theoretical knowledge related to the project. Skin regeneration, gene regulation will be mainly included. 2. Learn the principle and technical operation of the experiment and complete the experiment. Cell culture, transfection, real-time PCR, western blotting, immunofluorescent and data analysis will be included. 3. Learn and complete data analysis and statistics. It mainly includes data, image processing and statistical analysis.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No theoretical teaching provided
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student’s name will be mentioned in a future publication - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge and skills of biology
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
- Wang et al. PEI-coated Fe3O4 nanoparticles enable efficient delivery of therapeutic siRNA targeting REST into glioblastoma cells. International journal of molecular sciences; 19(8); 2230.doi:10.3390/ijms 19082230
- Zhang et al. Inhibition of rest suppresses proliferation and migration in glioblastoma cells. International journal of molecular sciences; 17(5); 664.doi: 10.3390/ijms17050664.
- Zhang et al. miR-136 modulates TGF-β1-induced proliferation arrest by targeting PPP2R2A in keratinocytes. BioMed Research International; 2015:453518. doi: 10.1155/2015/453518
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