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Investigating the Effects of Topiramate on Amygdala and Paraventricular Nucleus of Hypothalamus of Obese Rats
Turkey (TurkMSIC) - Okan University LC, Istanbul
Okan University Faculty Of Medicine, Histology & Embryology Department, Tepeoren Street, Tuzla Campus, 34959 Tuzla/Istanbul
Prof. Dr. Berrin Zuhal ALTUNKAYNAK,
Lecturer Isinsu ALKAN
Type of Research Project
- Basic science
What is the background of the project?
Please, provide the background of the project (Provide information about the project; min. 10 lines, max. 25 lines) The increasing prevalence of obesity in today’s world create the need of investigating obesity to a great extent and searching for cures. Antiepileptic drugs are being commonly preferred for the treatment of obesity patient because of the weight-loss effects. In this context, the question of how the usage of the antiepileptic drugs affect several brain areas and by virtue of what they cause to weight loss became extremely important. Although there are clinical studies regarding the effects, there are less researches done on the subject. Topiramate, which is one of the most commonly used agents especially in the treatment of morbidly obese patients, has been reported to increase weight loss considerably in the literature; however, the fact of no extensive research has been done necessitate conducting a new research project on the topic. This study will focus the effects of topiramate on obese subjects’ brain tissues.
What is the aim of the project?
Within the project, it is aimed to conduct a research on the regions on the brain, the amygdala and the paraventricular nucleus which are known as the regions involved in obesity and how they are affected under the usage of the antiepileptic drug topiramate by using stereological methods.
What techniques and methods are used?
In this project, obesity-modeled rats were injected topiramate 0.02 mg / kg intraperitoneally for 6 weeks and subjects' weight loss was recorded during the experimental period. At the end of the experimental procedure the subjects were anesthetized and underwent intracardiac perfusion and brain tissues were removed after the experimental period. The brain tissues were subjected to routine histological tissue processing and 20-micron thick sections were taken for stereological analysis. Calculation of neuron densities of amygdala and paraventricular nuclei were to be performed on the sections. Physical dissector method will be used to analyze neuron density. In the physical dissector method, the counting of the particles on serial sections taken from the subject, which are in one section and not in the other section is performed. One of the sections is considered as sample and one is considered as observation section; the particles in the sample section but not in the observation section are counted. The results of stereological analyzes will be evaluated statistically and interpreted. In the scope of the project, camera attachment light microscope (LM), brain tissue preparations, Cresyl violet stain (Cresyl Violet Acetate solution is used to stain Nissl substance in the cytoplasm of neurons in paraformaldehyde or formalin-fixed tissue.) will be used to ensure the visibility of the cells.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
What are the tasks expected to be accomplished by the student? (Minimum 10 lines, provide detailed explanation) - Sectioning 20 micron-thick sections from the brain blocks, and staining the samples with Cresyl violet dye in order to increase visibility of the cells. - To investigate the Rat Brain atlas in order to determine the amygdala and paraventricular areas on the prepared sections and to determine the areas under light microscope. - After the determination of areas, taking pictures of the sections with the help of the camera attached to the light microscope. - Performing physical dissector counts on the captured images using Image J program.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
At the beginning of the program, an informative course will be given to the students in order to convey the project in detail and specify the steps. In addition, presentation of the equipment to be used in the laboratory and conditions of use will be explained. The teaching session is planned to be approximately 4 hours and will be performed by Prof. Dr. Zuhal ALTUNKAYNAK and Lecturer Isinsu ALKAN.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No specific skills are required.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Pre-Medical students from the American-British system
- Isinsu Alkan; Berrin Zuhal Altunkaynak; Gamze Altun & Erkan Erener (2019) The investigation of the effects of topiramate on the hypothalamic levels of fat mass/obesity- associated protein and neuropeptide Y in obese female rats; Nutritional Neuroscience; 22:4; 243-252; DOI:10.1080/1028415X.2017.1374033
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