Projects
Name
Investigations on the selection of the most informative immunophenotypic markers for identifying the leukemic blasts to allow distinction with the normal blasts.
University
Belgium (BeMSA) - Ghent University, Ghent
Domain
Hematology
Departement
Department of Diagnostic Sciences
Head
Jan Philippé
Tutor
Jan Philippé
Languages
English, Dutch
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes No No No No
Type of Research Project
- Basic science
What is the background of the project?
Immunophenotyping of Acute Myeloid Leukemia (AML) samples is crucial for outcome prediction and treatment guidance in both adults and children. Optimizing analysis panels with more and more markers combined and thus running a high-dimensional panel not only provides deeper insights, but can also allow analysis of smaller sample size (as otherwise multiple tubes might be necessary), which is especially relevant for children. In addition, next to the specific AML panel under development in this project, our new software tools will also be very relevant for childhood leukemias in general.
What is the aim of the project?
The main goals are the selection of the most informative immunophenotypic markers for identifying the leukemic blasts which also allow distinction with the normal blasts. Special focus is on the leukemic stem cells and how they may be distinguished from the normal hematopoietic stem cells.
What techniques and methods are used?
1. First several scientific articles must be analyzed. 2. Thereafter a software package for analysis of (decoded) data files must be filled in for statistic research (SPSS). 3. Seeking optimal combinations of - Fluorochrome for each marker - Flow cytometric analysis - Making a profile where compensation issues in the analysis can be tackled.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
● The student must be willing to read a certain number of scientific articles. ● The student must follow the analysis procedure in the lab, in order to fully understand the technology and applicability of clinical flow cytometry. ● The student must be willing to explore a software package for analysis of (decoded) data files that he/she will be working with.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
● The student will be offered some literature to better understand the flow cytometric technique and the applicability in the analysis of acute myeloid leukemias. ● The student will be introduced to the use of software which is used to analyze the high-dimensional and complex fcs by his/her tutor. Those files are obtained by flow cytometric analyses.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student must be willing to explore a software package for analysis of (decoded) data files. (SPSS)
Are there any legal limitations in the student’s involvement
No
Hours
7
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- Students in biomedical fields
- Dental medicine students (IADS members)
Articles
- Leukaemic stem cell load at diagnosis predicts the development of relapse in young acute myeloid leukaemia patients. Hanekamp D. et al. Br J Haematol 2018; Nov; 183(3): 512-6.
- EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal; reactive and malignant leucocytes. Van Dongen J. et al. Leukemia 2012 Sep; 26(9): 1908-75.