To explore the role and mechanisms of Runx2 in the progression of Hepatocellular carcinoma
Taiwan (FMS-Taiwan) - Fu Jen University, Taipei
School of Medicine, Colledge of Medicine
Professor Dee Pei
Chih-Li Chen, Ph.D., Associated Professor
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Basic science
What is the background of the project?
Hepatocellular carcinoma (HCC) is the most lethal and prevalent type of liver cancers. HCC is a highly heterogeneous tumor that develops via activation of multiple pathways. Studies suggest that the complexity and clinical variability of HCC mainly depend on different molecular alterations that arise during the development and progression of this disease. MiRNAs are involved in multiple cellular processes including cell differentiation, stem cell maintenance, and epithelial– mesenchymal transition.MiR-196 is known to direct the cleavage of HOXB8 mRNA and also promote the osteogenic differentiation and osteopontin (OPN) production by inhibiting HOXC8 transcription. OPN play an important the HCC recurrence. Knockdown miR196 in HCC cell line, we observed the level of the osteogenic transcription factor and transcription activator of OPN, Runx2, was reduced. Overexpression of Runx 2 has been identified certain types of cancer. Thus, we hypothesize that miR196 may promote the progress of HCC through regulating the level of Runx2. In this proposal, we intend to explore the role and mechanisms of of Runx 2 in the progression of hepatocellular carcinoma.
What is the aim of the project?
Specific aim 1: to explore the mechanisms involved in miR196 regulating Runx2 expression in HCC cell line. Specific aim 2: To explore the biological role of Runx2 in the progression of hepatocellular carcinoma Specific Aim 3: to explore and characterize the role of downstream targets of Runx2 in the progress ion of HCC
What techniques and methods are used?
We will use the following biochemical, and molecular biological approaches to analyse the role of Runx2 in the development of HCC.Western blot and Real-time PCR , cell culture , establishment of shRNA-carrying cells, Ultralow attachment spheroid assay, Colony formation assay,invasion assay, transendothelial migration assay, In vivo tumorigenic and metastatic study, Immunohistochemistry.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will learn new techniques from the laboratory and enjoy the process of scientific discoveries. When performing experiments, the student is expected to follow the lab safety rules. If animal handling is needed for the project, the student is required to complete the animal handling training and follow all rules and protocols established by the Institutional Animal Care and Use Committee (IACUC). When the student is not in the lab, trying as many different Taiwanese cuisines is highly recommended.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic concepts on biology, chemistry, or physics are required. Experience in hands-on laboratory work is preferred.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- High Expression of MicroRNA-196a is Associated with Progression of Hepatocellular Carcinoma in Younger Patients.Wang SY; Chen CL; Hu YC; Chi Y; Huang YH; Su CW; Jeng WJ; Liang YJ; Wu JC. Cancers (Basel). 2019 Oct 13;11(10). pii: E1549. doi: 10.3390/cancers11101549.