Projects
Name
Behavioral tests and oxidative stress parameters in toxicological studies
University
Turkey (TurkMSIC) - Suleyman Demirel University, Isparta
Domain
Physiology
Departement
Suleyman Demirel University, Medical Faculty Department of Physiology, Cunur Mh., East Campus, 32200 Merkez/Isparta
Head
Assoc. Prof. Dr. Mustafa Saygin
Tutor
Assoc. Prof. Dr. Mustafa Saygin
Languages
Turkish, English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes No No No No No
Type of Research Project
- Basic science
What is the background of the project?
Please, provide the background of the project (Provide information about the project; min. 10 lines, max. 25 lines) Our department has been established in 1993. Oxidative stress markers are analyzed in our laboratory. Tissue homogenization is performed. Protein determination in tissue, MDA (Malondialdehyde), SOD (Superoxide dismutase), CAT (Computerized axial tomography), GPx (glutathione peroxidase) determinations are performed. Western blot is studied from protein determination method. DNA fractures can be examined by comet analysis. Water maze test is carried out. Physiology studies are important because it is an experimental scientific discipline and is of central importance in medicine and related health sciences. It provides a thorough understanding of normal body function, enabling more effective treatment of abnormal or disease states.
What is the aim of the project?
Please, provide the background of the project (Provide information about the project; min. 10 lines, max. 25 lines) Our department has been established in 1993. Oxidative stress markers are analyzed in our laboratory. Tissue homogenization is performed. Protein determination in tissue, MDA (Malondialdehyde), SOD (Superoxide dismutase), CAT (Computerized axial tomography), GPx (glutathione peroxidase) determinations are performed. Western blot is studied from protein determination method. DNA fractures can be examined by comet analysis. Water maze test is carried out. Physiology studies are important because it is an experimental scientific discipline and is of central importance in medicine and related health sciences. It provides a thorough understanding of normal body function, enabling more effective treatment of abnormal or disease states.
What techniques and methods are used?
What techniques and methods are used? Mention the steps/stages in this project. (Provide examples and avoid abbreviations; which tools will be used in general in this project?) Analysis of oxidant-antioxidant enzymes, DNA damage analysis with alkali comet and western blot. Oxidative stress parameter of tissue oxidant-antioxidant balance will be studied after the application of tissue disintegration (homogenization) process to be used in our project. The determination of the protein to be carried out within the scope of the project will be done by western blot in order to measure the amount in the tissue. DNA fractures will be examined in the study blood samples by comet assay. All this work will be routinely performed for toxicology studies. If the tissue to be used in the study is brain hippocampus, behavioral test will be performed with morris water maze. After cutting, the above protocol will proceed. From the beginning to the end of the study, the assistants in the department are actively working at all stages from animal care, drug use, testing to the last section.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
What are the tasks expected to be accomplished by the student?
What techniques and methods are used? Mention the steps/stages in this project. (Provide examples and avoid abbreviations; which tools will be used in general in this project?) Analysis of oxidant-antioxidant enzymes, DNA damage analysis with alkali comet and western blot. Oxidative stress parameter of tissue oxidant-antioxidant balance will be studied after the application of tissue disintegration (homogenization) process to be used in our project. The determination of the protein to be carried out within the scope of the project will be done by western blot in order to measure the amount in the tissue. DNA fractures will be examined in the study blood samples by comet assay. All this work will be routinely performed for toxicology studies. If the tissue to be used in the study is brain hippocampus, behavioral test will be performed with morris water maze. After cutting, the above protocol will proceed. From the beginning to the end of the study, the assistants in the department are actively working at all stages from animal care, drug use, testing to the last section.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Will there be any theoretical teaching provided for the student (preliminary readings, lectures, courses, seminars)? Please describe how much teaching and by whom. We will practice some analyses methods by theoretical and practical. The theoretical and practical lessons will be given 2-4 times a week by Assoc. Prof. Dr. Mustafa Saygin.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No specific skills are required.
Are there any legal limitations in the student’s involvement
No
Hours
8
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
Articles
- For the use of students considering participating in the project; further information can be found from the following articles (Please add at least one specific article that is not a book; with correct reference) “The impact of methotrexate on lung inflammatory and apoptotic pathway biomarkers-The role of gallic acid.” Saygin M.; Ozturk O.; Ozmen O.; Ilhan I.; Gonca T.; Gumral N.; Orhan H.; Aslankoc R. . https://www.ncbi.nlm.nih.gov/pubmed/27876213