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Analysis of Recurrent Tuberculosis in Cukurova Region of Turkey
Turkey (TurkMSIC) - Cukurova University, Adana
Cukurova University, Faculty of Medicine, Medical Microbiology Department, 01330 Balcali,Saricam, Adana,TURKEY
Prof. Dr. Fatih KOKSAL
Prof. Dr. Fatih KOKSAL
Type of Research Project
- Basic science
What is the background of the project?
Recurrent Tuberculosis (TB) is one of the major problem for the management of correct and effective treatment period. Repetitive episodes of TB patients who have previously been treated and were declared as completed their treatment or got cured are considered as recurrence of TB. Recurrence can be either a relapse due to endogenous reactivation with the same strain of Mycobacterium tuberculosis (Mtb) or reinfection caused by new TB bacilli. This is the retrospective population based study in Cukurova Region of Turkey based on Mycobacterial Interspersed Repetitive Units- Variable Number of Tandem Repeat (MIRU-VNTR).
What is the aim of the project?
Estimate the incidance of recurrence Identify the associated risk factors Estimate the proportion of relapse and reinfection Determination of bacteriological cure rate in TB patients
What techniques and methods are used?
Collection of TB notifications from 2015-2019 with the help of the Turkey General Directorate of Public Health Database. DNA will be extracted from positive culture by using mechanical extraction method. For Mycobacterial Interspersed Repetitive Units- Variable Number of Tandem Repeat (MIRU-VNTR) and spoligotyping methods polymerase chain reaction will be performed with different pairs of primers. After polymerase chain reaction size of amplicons will be examined by agarose gel electrophoresis and membrane hybridization for MIRU-VNTR and spoligotyping respectively. Following the performed techniques results will be evaluated by using different types of softwares as NCSS 2019 (National Council of Social Service) and SPSS 26 (Statistical Package for Social Sciences).
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The student will be expected to perform the following tasks: -Mycobacterium tuberculosis diagnosis in clinical samples from Tuberculosis notifications between 2015-2019 (which will be collected by our research team) -Participate in the process of DNA amplification through polymerase chain reactions and interpretation of those polymerase chain reaction’s results. -The student will also search for possible correlations between the patient samples using various softwares as NCSS 2019 (National Council of Social Service) and SPSS 26 (Statistical Package for Social Sciences) and analyse the long-term and short-term surveillance study methods.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student will be asked to participate to regular lab meetings where he/she may either present her own studies or present scientific articles to be discussed. Also theoretical teaching about routin procedure of Mycobacterium tuberculosis complex diagnosis, analysis of long-term and short-term surveillance study methods will be provided for the student and he/she will gain experience about informatics and analysis in tuberculosis infections. The theoretical teaching will be provided according to the availability of Prof.Dr. Fatih KOKSAL at least once a week.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No spesific skills are required.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- P. Supply; C. Allix; S. Lesjean; M. Cardoso-Oelemann; S. Rüsch-Gerdes; E. Willery; E. Savine; P. De Haas; H. Van Deutekom; S. Roring; P. Bifani; N. Kurepina; B. Kreiswirth; C. Sola; N. Rastogi; V. Vatin; M.C. Gutierrez; M. Fauville; S. Niemann; R. Skuce; K. Kremer; C. Locht; D. Van Soolingen; Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis; J. Clin. Microbiol. 44 (2006) 4498–4510. doi:10.1128/JCM.01392-06.
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