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Basil’s (Ocimum Gratissimum) protective effect on hippocampus neuron loss and reflection on spatial memory behavior tests, in streptosotocin-induced experimental Alzheimer’s Disease, on rats
Turkey (TurkMSIC) - Pamukkale University, Denizli
Department of Anatomy, Pamukkale University Morphology Building 20070 Kinikli/Pamukkale/Denizli
Prof. M. Bulent OZDEMIR, MD
Prof. M. Bulent OZDEMIR, MD
Type of Research Project
- Basic science
What is the background of the project?
Alzheimer’s Disease is the most common cause of dementia. Nowadays, research is being carried out about the pathophysiology of the disease and treatment modalities associated with the mechanism of the disease is being developed. The antidiabetic effect of basil (Ocimum Gratissimum) extract through Glut4 is being shown via researches. This mechanism is on the same pathway with pathophysiology of Alzheimer’s disease. To receive a diagnosis of Alzheimer's, the person must have experienced a decline in cognitive or behavioral function and performance compared with how they were previously. This decline must interfere with their ability to function at work or in usual activities.
What is the aim of the project?
Analysing Basil’s neuroprotective effect on STZ induced Alzheimer’s Disease in rats’ hippocampi’s and Basil’s effect on spatial memory behavior
What techniques and methods are used?
1. Constitution of experimental groups 2. Preparation of sham groups 3. Intraperitoneal application of 0,6 mg streptozotocin to streptozotocin(STZ) group 4. Intraperitoneal application of 0,6 saline to sham group 5. Constitution of STZ+Basil group 6. Constitution of saline+Basil group 7. Urine and Blood Tests 8. Performing Watermaze Behavior Test 9. Preparation of brain fraction through decapitation and frozen, Hematoxilene&Eozin dye application 10. Cell count using stereologic procedures 11. Determination of acetylcolin quantities in brain cells 12. Collation of findings using statistical methods.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
We expect from the incoming student/s that they; 1.Prepare solutions necessary for the research, 2.Observe of steps that are mentioned previously, 3.Read papers and textbook and expand their knowledge on Neuroanatomy and Alzheimer’s Disease, 4. Histological staining techniques 5.Observing Intraperitoneal application of 0,6 mg streptozotocin to streptozotocin(STZ) group 6.Learning Watermaze Behavior test Techniques 7.Learning cell culture protocols 8. Observing urine and blood tests and anaylzes 9.Learning cell counting techniques and formules 10. Preparation of brain fraction through decapitation and frozen 11.Learning many types bacteria structure and mechanisms for experiments
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The following topics will be provided in the form of seminars by Prof. M. Bulent OZDEMIR Lessons will be provided according to the availability of the teacher, at least once per week: -Cell Cycle -Cell Culture Protocols -Urine, blood tests analyzes -Azlheimer’s Disease mechanisms
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No specific skills are required.
Are there any legal limitations in the student’s involvement
Students will not be permitted to handle the lab rats.
Type of students accepted
This project accepts: - Medical students
- Lane EM; Hohman TJ; Jefferson AL(2016). Insulin-like growth factor binding protein-2 interactions with Alzheimer’s disease biomarkers. Springer Science+Business Media. New York DOI 10.1007/s11682-016-9636-0
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