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Functional study of candidate disease causative genes for congenital heart disease in zebrafish
China (IFMSA-China) - Wuhan University School of Medicine, Wuhan
Department of Biochemistry and Molecular Biology
Type of Research Project
- Basic science
What is the background of the project?
Congenital heart disease is one of the most common birth defects, yet the genetic mechanism is still not completely understood. Many candidate disease causative genes have been discovered clinically, and funcational studies are urgently needed. Zebrafish is an excellent model animal to study early development, owing to its facile husbandry and optical transparency of its larve. In this project, we will focus on a certain candidate disease causative gene of congenital heart disease, and apply CRISPR/Cas9 techonology to generate zebrafish mutant. By mutating such a gene, we can observe the phenotype of the mutant zebrafish and explore the function of this gene in the heart development.
What is the aim of the project?
To find out the function of a certain candidate disease causative gene of congenital heart disease
What techniques and methods are used?
1) Search for a candidate disease causative gene of congenital heart disease 2) Design target sites and synthesize gRNA 3) Inject the gRNA into zebrafish embryo 4) Verify the efficiency of gene editing 5) Observe the phenotype of the mutant zebrafish
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The student is expected to carry out the whole project. At first, the student need to read revelant literature to search for a candidate disease causative gene of congenital heart disease. Then, a sequence of oligonucleotide need to be synthesized as the sgRNA. After that, the student will inject the sgRNA into zebrafish embryo and verify the efficiency of the gene editing. Finally, the student will observe the heart development of the mutant zebrafish and draw a conclusion.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Student will all the skills needed when doing the project.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Student should know how to read literature critically.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Pre-Medical students from the American-British system - Students in biomedical fields
- Asnani A and Peterson RT. The zebrafish as a tool to identify novel therapies for human cardiovascular disease. Disease Models & Mechanisms (2014) 7:763-767
- Grant MG; Patterson VL; Grimes DT; Burdine RD. Modeling Syndromic Congenital Heart Defects in Zebrafish. Current Topics in Developmental Biology (2017) 124:1-40.
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