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Investigate the effect of K562 cell receptors on signal transduction pathways and cell division in K562 cells
Turkey (TurkMSIC) - Marmara University, Istanbul
Biophysics and Medical Physics
Department of Biophysics ,Marmara University, Faculty of Medicine, Basibuyuk Mh., 34854, Istanbul, Turkey
Prof. Dr. Hulya Cabadak
Prof. Dr. Hulya Cabadak
Type of Research Project
- Basic science
What is the background of the project?
This project includes studyings to detect signal transduction through receptors in cancer. Cancer is driven by genetic and epigenetic alterations that allow cells to overproliferate and escape mechanisms that normally control their survival and migration. Many of these alterations map to signaling pathways that control cell growth and division, cell death, cell fate, and cell motility. Cancer-associated events proceed in distinct receptors in distinct ways. K-562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognizable progenitors of the erythrocytic, granulocytic and monocytic series. In Turkey, about 163 thousand new cancer cases a year are diagnosed according to the latest datas.
What is the aim of the project?
To investigate and understand K-562 cells signal transduction pathways in cancer.
What techniques and methods are used?
In order to understand how cancer cells work we use cell culture. Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. Enzyme-linked immunosorbent assay, also called ELISA or EIA is used to detect antibodies in using sample related to cancer. The assay uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology. Western blot analysis. The western blot is a widely used analytical technique in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. Western Blotting technique has 3 mainly step. -Protein Denaturation -Gel electrophoresis. -Transfer and imaging
What is the role of the student?
- The student will mainly observe
What are the tasks expected to be accomplished by the student?
-Learning cell culture protocols. Freezing, thawing and passaging. -Learning Western Blotting steps and analyses -Creating cell lines for experiment -Learning protein denaturation techniques -Learning to used kits for protein, DNA (Deoxyribonucleic acid) or RNA (Ribonucleic acid) isolation -Prepare different percentages of gel for electrophoresis -Learning cell-signal networks mechanisms -Observing ELISA steps and learning specific antibody-antigen recognition - Learning to use Microsoft Excel and Statistical Package for Social Sciences 26th edition for compiling and analyzing data
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There are many different subjects open for understanding methods. These subject will be taught -Cell- culture techniques protocols -Western Blotting Protocols and analyses -Cancer types -Cancer stem cell structure and properties -Cell cycle -Replication, Transformation, Translation and RNA, Protein İsolation techniques - Cell-signaling networks - Cell Homeostasis By Prof. Dr. Hulya Cabadak ( with preliminary readings) These courses will be given at least once per week.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No specific skills are required.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Students in biomedical fields
- Investigation of the Roles of Non-neuronal Acetylcholine in Chronic Myeloid Leukemic Cells and their Erythroid or Megakaryocytic Differentiated Lines. Aydin B; Cabadak H; Goren M.Z. Anticancer Agents Med Chem. 2018;18(10):1440-1447. doi:10.2174/1871520618666180406123154.
- The role of intracellular pathways in the proliferation of human K562 cells mediated by muscarinic receptors. Aydin B; Kan B; Cabadak H. Leuk Res. 2013 Sep;37(9):1144-9. doi:10.1016/j.leukres.2013.05.018. Epub 2013 Jun 22.
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