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Acquainting with the use of Clinical Microbiology as a Field and Tool in Modern Research
Turkey (TurkMSIC) –Sütçü İmam University LC, Kahramanmaraş
Kahramanmaras Sutcu Imam University Research and Practice Hospital the Department of Microbiology and Clinical Microbiology Avsar Mah. Batı Cevreyolu Blv. No: 251/A 46040 - Onikisubat/Kahramanmaras/Turkey
Prof.Dr. Murat ARAL
Assoc. Prof. Dr. Kezban Tulay YALÇINKAYA
Type of Research Project
- Basic science
What is the background of the project?
The Department of Microbiology and Clinical Microbiology of the Kahramanmaras Sutcu Imam Faculty of Medicine was established in 1999. Routine laboratory services are provided in the Kahramanmaras Sutcu Imam University Research and Practice Hospital. In addition, our department offers undergraduate, graduate and specialist trainings. Microbiology is a science called microorganism that examines small living things, many of which can only be seen under a microscope. Many of these microorganisms cause diseases in humans. At the beginning of these; bacteria, viruses, fungi and parasites. By examining these microorganisms in our laboratory, it is possible to determine what diseases occur in people. Turkish Society of Microbiology, founded in 1882, organizes seminars and conferences that aim to educate microbiologists on newly emerging bacteria and bacteriophage identification methods.The Turkish Society of Microbiology have many different group ;Forensic Medicine Microbiology Working Group,Anaerop Working Group,Standardization of Antibiotic Susceptibility Tests (ADTS) Working Group,Food and Waterborne Pathogens Working Group .
What is the aim of the project?
To reveal diseases caused by microbes, viruses, parasites and more, to find out what is responsible for a disease or symptom, to propose medication or strategy for treatment according to it, to take measures to protect other people from these diseases.
What techniques and methods are used?
PCR (polymerase chain reaction): PCR is shorthand for a simple but very useful procedure in molecular biology called the polymerase chain reaction. It is a technique used to amplify a segment of DNA of interest or produce lots and lots of copies. In other words, PCR enables you to produce millions of copies of a specific DNA sequence from an initially small sample – sometimes even a single copy. It is a crucial process for a range of genetic technologies and, in fact, has enabled the development of a suite of new technologies. PCR mimics what happens in cells when DNA is copied (replicated) prior to cell division, but it is carried out in controlled conditions in a laboratory. The machine that is used is simply called a PCR machine or a thermocycler. Test tubes containing the DNA mixture of interest are put into the machine, and the machine changes the temperature to suit each step of the process. ELISA (enzyme-linked immunosorbent assay): is a plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones. Other names, such as enzyme immunoassay (EIA), are also used to describe the same technology. In an ELISA, an antigen must be immobilized on a solid surface and then complexed with an antibody that is linked to an enzyme. Detection is accomplished by assessing the conjugated enzyme activity via incubation with a substrate to produce a measurable product. The most crucial element of the detection strategy is a highly specific antibody-antigen interaction Indirect Immunofluorescence Assay (IFA): IFA is one of the two most commonly used tests for antinuclear antibodies. Typically, HEp-2 cells are used as a substrate to detect the antibodies in human serum. HEp-2 cells are currently one of the most common substrates for antinuclear antibody detection by IFA. Quantitative nephelometry is a lab test to quickly and accurately measure levels of certain proteins called immunoglobulins in the blood. Immunoglobulins are antibodies that help fight infection. This test specifically measures the immunoglobulins IgM, IgG, and IgA.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
What are the tasks expected to be accomplished by the student?
The following tasks will be expected to be accomplished by the student: -At the end of the exchange the student should be adept at properly following lab protocols. -Examining the PCR (Polymerase Chain Reaction) method, ELISA method (enzyme-linked immunosorbent assay) and Indirect Immunofluorescence Assay (IFA) method and its steps while these methods are going to be used in the laboratory. -Analyzing the Quantitative nephelometry results with the help of the tutor -Following the steps and protocols of isolating, Cloning, and Sequencing DNA.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Prof.Dr. Murat ARAL will be providing lessons twice weekly on: - Get educated concerning basic immunology. -The structure and functions of immunoglobulins, especially IgG. - antigen,antibody detection and relations. -Isolation, Cloning, and Sequencing DNA mechanisms -The measurement of certain proteins called immunoglobulins in the blood via serological tests such as Quantitative nephelometry Please note that schedules may change depending on the availability of the tutor.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No specific skills are required. Student must have finished 2nd year of medical studies.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Clinical Microbiology Made Ridiculously Simple; 6th Edition Authors: Mark Gladwin; Bill Trattler and C. Scott Mahan
- Microbiology: An Introduction; 13th Edition Authors: Gerard J. Tortora; Berdell R. Funke and Christine L. Case
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