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Investigation of Innate lymphoid and MAIT cells in the cord blood of pregnant mothers with intrauterine growth retardationroject
Turkey (TurkMSIC) - Erciyes University, Kayseri
Department of Medical Biology, Faculty of Medicine Genom & Stem Cell Center Erciyes University,38030 Melikgazi, Kayseri, TURKEY
Dr. Ahmet Eken
Dr. Halit Canatan
Type of Research Project
- Basic science
What is the background of the project?
Innate lymphoid cells (ILCs) are immune cells belong to the lymphoid lineage. ILCs do not express antigen-specific receptors. ILCs have important functions in innate immune responses to infectious microorganisms and in the regulation of homeostasis and inflammation. Recently, a population of unconventional T cells named mucosal-associated invariant T (MAIT) cells are discovered. Intrauterine growth retardation (IUGR) refers to a condition where an unborn baby is smaller than it should be.Our project is focused on investigating ILC and MAIT cells in the cord blood of pregnant mothers with IUGR.
What is the aim of the project?
The goal of the project is to analyse the ILC, MAIT, and Follicular T Helper (TFH) cells in pregnancies who present with intrauterine growth retardation (IUGR). We plan to include pregnant mothers with morbidly adherent placenta (MAP), Hypertension, and diabetes mellitus.
What techniques and methods are used?
Cord blood will be drawn from the healthy pregnant donors, or IUGR mothers. Peripheral blood mononuclear cells will be isolated via ficoll Paque. Then MAIT, TFH and ILC cell populations will be anlaysed by Flow cytometric analysis after cell surface staining. Additionally, Cells will be stimulated with Propidium Monoazide (PMA) and Ionomycin and the production of signature cytokines for MAIT cells will be assessed. The techniques we will use are; Cell surface staining Flow Cytometry Flow Jo analysis Perihperal Blood Mononuclear Cell (PBMC) isolation
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
What are the tasks expected to be accomplished by the student?
- Observe and work with the PhD students during cord blood acquisitions - Learn and perform the isolation of PBMC’s using flow cytometry LSR 3 (under supervision) - Learn and perform pre-microscopic intracellular staining with PMA and Ionomycin flourescent dyes - Observe and help under supervision with the compiling and anaylsis of the project data usign FlowJo and Statistics Package for Social Science 26th edition.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Journal Clubs, Case Discussions by Dr. Ahmet Eken and Dr. Halit Canatan once a week.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No spesific skill required.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Eken A; Cansever M; Somekh I; Mizoguchi Y; Zietara N; Okus FZ; Erdem S; Canatan H; Akyol S; Ozcan A; Karakukcu M; Hollizeck S; Rohlfs M; Unal E; Klein C; Patiroglu T. Genetic Deficiency and Biochemical Inhibition of ITK Affect Human Th17; Treg; and Innate Lymphoid Cells. J Clin Immunol. 2019 May;39(4):391-400. doi: 10.1007/s10875-019-00632-5. Epub 2019 Apr 25. PubMed PMID: 31025232.
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