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Investigation of Neuroprotective and Curative Effects of Campherol Therapy in Transgenic Mouse Model of Alzheimer's Disease
Turkey (TurkMSIC) - Bezmialem University, Istanbul
Bezmialem Vakif University, Department of Medical Biology, Adnan Menderes Bulvari, Vatan Caddesi 34093 Fatih/Istanbul,TURKEY.
Asst. Prof. Birsen ELIBOL
Asst. Prof. Birsen ELIBOL
Type of Research Project
- Basic science
What is the background of the project?
Alzheimer's disease is the most common neurodegenerative disease in the elderly people. Dementia and loss of cognitive functions negatively affect the lives of not only patients but also their relatives. Apart from symptomatic therapies, no progress has been made yet, such as stopping the progression of the disease, regeneration, and recovering lost functions. Efforts to develop amyloid plaque-clearing agents, such as vaccines, antibodies, continue, but these therapeutics inhibit the progression of neuronal damage, but are insufficient for neuronal restructuring activities. Reactive oxygen species and free radicals are known to contribute to the pathophysiology of Alzheimer's disease. Amyloid plaques formed in the intercellular space are rich in metal and contribute to amyloid beta (Aβ) toxicity. Campherol, a flavonoid molecule, is a powerful antioxidant with metal capture properties. Because of this feature, it is a potential molecule for partial treatment of Alzheimer's disease. Few studies in the literature have shown that campherol provides cognitive recovery in Alzheimer's disease through its metal sweep feature. However, the effects of campherol on neurogenesis have not been studied and the molecular mechanisms of the healing process have not been elucidated. In Turkey there is a society called “Turkish Alzheimer Society” that is established in 1997 and does various actions on Alzheimer Disease. Based on the data of this society, in Turkey there are more than 600.000 families struggling with Alzheimer Disease and %8,7 of the Turkish citizens have Alzheimer Disease.
What is the aim of the project?
The aim of the project is to analize the effect of Campherol which is a potential molecule for partial treatment on cognitive recovery in Alzheimer’s disease.
What techniques and methods are used?
The following techniques are commonplace in the project: - Real time polymerase chain reaction (RT-PCR;used to amplify-copy small segments of deoxyribonucleic acid -DNA- using sequential thermal lysis and DNA polymerase enzymes) , - Western Blot gel electroporesis (used to detect specific protein molecules from among a mixture of proteins by forcing them across an electrically charged gel) , - Immunofluorescence staining (antigen-detection test that is used primarily on frozen tissue sections, cell “smears,” or cultured cells. Uses artificially produced immunoglobulins that target specific antigens and produce fluorescence under ultraviolet light) The following are the behavioral tests used on animal specimens: - Rotarod test in which animals are tested for balance on a rotating rod, - Plus maze and morris water maze tests for cognition testing - Passive avoidance, forced swim test, tail suspension tests for self preservation and instictive behavior anaylses.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The main duty of the student is observing. He/she may help the questionnaire distribution and collecting. May help to data record. The student may help to analyzing data using computerized aides. Familiarity with basic computer science would be helpful for fast adaptation. In a time, the student may take active part in the practical aspects of a piece of lab work. The student will observe the animal experiments but can not take part in the experiments because of the legal limitations. The student will be involved to laboratory work as well-microscopy, sample preparation, polymerase chain reaction and blotting. The student will also be required to perform everday laboratory tasks such as preparing slides and analyzing data. The student will participate to all experiments and observations. The student will be expected to prepare a report about “Investigation of Neuroprotective and Curative Effects of Campherol Therapy in Transgenic Mouse Model of Alzheimer’s Disease”. Most of said work will be carried out alongside the faculty staff. The student will receive instructions regarding the usage of Statistical Package for Social Sciences 26th edition from the staff, afterwards they will also take part in the analysis of the obtained data.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There will be preliminary theoretical teachings on the everyday techniques used in the laboratory such as sample preparation, polymerase chain reaction, blotting etc. before laboratory work starts. The teaching will be provided at least once per week by Asst. Prof. Birsen ELIBOL depending on the availibility of the teacher.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
If the student wishes to actively take part in animal experiments they must present a Certification of Experimental Animal Usage obtained from their countries of origin or from Turkey. Otherwise they can just observe the animal parts and be involved with the rest of the project.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
- Manoharan S; Guillemin GJ; Abiramasundari RS; Essa MM; Akbar M; Akbar MD; The role of reactive oxygen species in the pathogenesis of Alzheimer’s disease; Parkinson’s disease and Huntington disease; a mini review. Oxid Med Cell Longev. (2016).
- Somayeh Kouhestani; Adele Jafari; Parvin Babaei. Kaempferol attenuates cognitive deficit via regulating oxidative stress and neuroinflammation in an ovariectomized rat model of sporadic dementia (2018).
- Liu M; Guo H; Li C; Wang D; Wu J; Wang C; Xu J; Qin RA; Cognitive improvement of compound danshen in an Aβ25-35 peptide-induced rat model of Alzheimer's disease; BMC Complement Altern Med.; Oct 23;15:382;( 2015)
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