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Development of multivalent vaccine against avian influenza viruses
Korea (KMSA) - Korea university, Seoul
Department of Microbiology
Type of Research Project
- Basic science
What is the background of the project?
Virus laboratory in the Microbiology department has previously generated several recombinant Newcastle disease virus (rNDV) expressing foreign proteins, including the influenza virus hemagglutinin (HA) gene. This rNDV is derived from a live attenuated strain used for poultry vaccination. NDV itself has inherent adjuvant properties in humans because it is a potent inducer of both human interferon /(IFN-/) production and dendritic cell maturation. Accordingly, rNDV vaccine vector may lead to induce strong humoral and cellular immunity and bestowing cross-protection without the need of an adjuvant in humans. Therefore, rNDV vaccine vector will be useful to develop vaccines for human pathogens like Ebola, Middle East Respiratory Syndrome-Corona virus (MERS-CoV), Nipah virus, and Hantavirus.
What is the aim of the project?
The purpose of this project is to develop the multivalent vaccines against avian influenza viruses based on reverse genetics system of Newcastle disease virus.
What techniques and methods are used?
Virus laboratory in the Microbiology departmenthas established the reverse genetics system for the commercial vaccine strains. By using reverse genetics techniques, foreign genes like the hemagglutinin of a highly pathogenic avian influenza (HPAI) virus can be introduced into NDV genomes. Thus, students will be experienced on the molecular cloning work to generate the recombinant viruses using reverse genetics system. As a next step, animal study will be performed to evaluate the vaccine efficacy by immunizing a vaccine. In this animal study, Balb/C mouse will be used.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
(1) The molecular cloning technology of the genome (2) Next generation sequencing technology (3) Bioinformatics technology (4) Protein expression by western blot or immunofluorescence assay (5) Vaccine evaluation in the Balb/C mouse (6) Statistical analysis
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Students will have the opportunity to present their data in laboratory meeting. In addition, journal club is held in every week at the department of microbiology. Students will join this journal club and discuss with laboratory members about thecurrent vaccine research.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student should be accustomed to freely handle mouse. In this mouse experiment, students will have an experience to inject vaccine antigen by intramuscular route. Students do not need to feed the mouse or change bedding or cages.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Students in biomedical fields - Dental medicine students (IADS members)
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