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Anti-cancer drug and chemoprotectant from Piper nigrum
Thailand (IFMSA-Thailand) - Faculty of Medicine, Songkla University, Songkhla
Assoc. Prof. Paramee Thongsuksai
Assist. Prof. Dr. Potchanapond Graidist
Type of Research Project
- Basic science
What is the background of the project?
Previously, we showed that piperine free Piper nigrum extract (PFPE) demonstrated potent cytotoxic effects on many cancer cell lines, especially breast cancer. PFPE induced cell cycle arrest and apoptosis. Moreover, the finding of cytotoxicity and slow growth rate of tumor incidence following a single oral administration of PFPE in female Sprague-Dawley rats with NMU-induced mammary tumors led to the concept that PFPE might play a role in breast cancer prevention and treatment. Nowadays, we further explore the effect of PFPE on other cancer including cholangiocarcinoma and colon cancers. We also work in an active pure compound from PFPE, namely kusunokinin, and find the targeted protein in vitro and in vivo. Kusunokinin, a lignan compound, inhibited many cancer type such as breast, colon, cholangiocarcinoma and lung cancer cell lines. This compound suppressed breast tumor growth in rat without side effects. Therefore, clearly understanding the specific action of PFPE and kusunokinin are required for chemoprotectant and anticancer drug development.
What is the aim of the project?
1. To investigate the mechanism of PFPE on chemoprevention. 2. To identify target protein of kusinokinin and its derivatives.
What techniques and methods are used?
For the first objective, RAW264.7 (macrophage) and cancer cells will be treated with PFPE and treated cell will be harvested to determine protein of interests using Western blot analysis. For the second objective, specific target proteins of kusinokinin and its derivatives will be identified using computational methods including Molecular docking technique. Then, cancer cells will be treated with kusinokinin and derivatives. Cell will be harvested and target or related proteins will be detected using Western blot analysis.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Student will be able to perform many experiments including Molecular docking, cell culture and Western blot analysis. All tasks will be supervised by our lab members. The student is expected to discuss his/her results or overall understandings of the project with post-doctor and Ph.D. student. If student chooses to work in their own project, they will do an easy experiment such as MTT assay, Molecular docking. Due to each experiment take long time, student can work only 1 experiment for 4 weeks and more than 1 experiment for 8 weeks. However, student can work in many experiments, if his/her works with tutor (2 experiments for 4 weeks and more than 2 experiments for 8 weeks).
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Previous publications of our work will be provided. The lecture will be arranged if required. Student will be attended seminar of Biomedical Sciences program, this seminar run for Master-Ph.D. student.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student’s name will be mentioned in a future publication - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Students should have knowledge/laboratory skill in Life Science or Biomedical Science or Molecular biology.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
- Sriwiriyajan S; Tedasen A; Lailerd N; Boonyaphiphat P; Nitiruangjarat A; Yan D; Graidist P. 2016. Anti-cancer and cancer preventive effects of a piperine free Piper nigrum extract on N-nitrosomethylurea induced mammary tumorigenesis in rats. Cancer Prev Res. 9(1):74-82.
- Deng Y; Sriwiriyajan S; Tedasen A; Hiransai P; Graidist P. 2016. Anti-cancer effects of Piper nigrum via inducing multiple molecular signaling in vivo and in vitro. J Ethnopharmacol. 188:87-95.
- Tedasen A; Choomwattana S; Graidist P; Tipmanee V. 2017. Structure-guided cancer blockade between bioactive bursehernin and proteins: Molecular docking and molecular dynamics study. J Mol Graph Model. 74:215-224.
- Sriwiriyajan S; Sukpondma Y; Srisawat T; Madla S; Graidist P. 2017. (-)-Kusunokinin and piperloguminine from Piper nigrum: An alternative option to treat breast cancer. Biomed Pharmacother. 92:732-743.
- Rattanaburee T; Thongpanchang T; Wongma K; Tedasen A; Sukpondma Y; Graidist P. 2019. Anticancer activity of synthetic (±)-kusunokinin and its derivative (±)-bursehernin on human cancer cell lines. Biomedicine & Pharmacotherapy. 117: 109115.
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